Ohashi Atsushi, Nakatani Masashi, Hori Hideo, Nakai Shigeru, Tsuchida Kunihiro, Hasegawa Midori, Tsuboi Naotake
Faculty of Clinical Science, School of Medical Sciences, Fujita Health University, Toyoake, Japan.
Faculty of Rehabilitation and Care, Seijoh University, Tokai, Japan.
Ther Apher Dial. 2023 Dec;27(6):1023-1027. doi: 10.1111/1744-9987.14047. Epub 2023 Aug 19.
Indoxyl sulfate (IS) is a protein-bound uremic toxin that causes uremic sarcopenia. IS has poor dialysis clearance; however, the addition of a binding competitor improves its removal efficiency.
Dialysis experiments were performed using N-acetyl-l-tryptophan (L-NAT) instead of l-tryptophan (Trp) using pooled sera obtained from dialysis patients. The molecular structures of L-NAT and Trp were similar to that of IS. Therefore, we examined whether Trp and L-NAT were involved in muscle atrophy in the same manner as IS by performing culture experiments using a human myotube cell line.
The removal efficiency of L-NAT was the same as that of Trp. However, L-NAT concentrations in the pooled sera increased at the end of the experiment. Trp (1 mM) decreased the area of human myocytes, similar to IS, whereas L-NAT did not.
L-NAT is a binding competitor with the ability to remove protein-bound IS while preventing sarcopenia.
硫酸吲哚酚（IS）是一种与蛋白质结合的尿毒症毒素，可导致尿毒症性肌肉减少症。IS的透析清除率较低；然而，添加一种结合竞争剂可提高其清除效率。
使用从透析患者获得的混合血清，用N-乙酰-L-色氨酸（L-NAT）代替L-色氨酸（Trp）进行透析实验。L-NAT和Trp的分子结构与IS相似。因此，我们通过使用人肌管细胞系进行培养实验，研究Trp和L-NAT是否与IS以相同方式参与肌肉萎缩。
L-NAT的清除效率与Trp相同。然而，实验结束时混合血清中L-NAT的浓度增加。Trp（1 mM）与IS相似，可减小人肌细胞的面积，而L-NAT则不会。
L-NAT是一种结合竞争剂，具有清除与蛋白质结合的IS并预防肌肉减少症的能力。
