College of Chemistry and Chemical Engineering, Jiangxi Normal University, 99 Ziyang Road, Nanchang, 330022, China; Key Laboratory of Evaluation of Traditional Chinese Medicine Efficacy (Prevention and Treatment of Brain Disease with Mental Disorders), Jiangxi University of Chinese Medicine, 1688 Meiling Road, Nanchang, 330004, China.
College of Chemistry and Chemical Engineering, Jiangxi Normal University, 99 Ziyang Road, Nanchang, 330022, China.
Biosens Bioelectron. 2023 Oct 15;238:115593. doi: 10.1016/j.bios.2023.115593. Epub 2023 Aug 11.
In view of the importance of quantification of neuron-specific enolase (NSE), an electrochemical NSE immunosensor was developed. The sandwich voltammetric immunosensor utilized vinyl-functionalized crystalline covalent organic framework (COFTAPT-Dva) modified electrode to load lots of Ab1 via thiol-ene "click" reaction as matrix. A crystalline cationic EB-COF:Br was used to load Au nanoparticles (AuNPs) and H3[PMo12O40] (PMo12) as immunoprobe. The AuNPs with the size of about 30 nm were firstly grown on EB-COF:Br and then a large number of electroactive PMo12 were uniformly assembled on AuNPs/EB-COF:Br via ion exchanging reaction. The AuNPs not only facilitated the bonding of Ab2 based on Au-S bond, but also improved performance of Ab2/AuNPs/EB-COF:PMo12 immunoprobe. The sensitivity of sandwich electrochemical immunosensor could be primarily amplified based on loaded abundant PMo12. Secondary sensitivity amplification of immunosensor could be achieved by using PMo12 to catalyze ascorbic acid. The linear range of sandwich voltammetric immunosensor based on current change of differential pulse voltammetry is 500 ± 36 fg mL - 100 ± 8 ng mL. Thanks to the dual sensitivity amplification strategy, the sensitivity is as high as 54.06 ± 3.2 μA cm/lg(cNSE/ng mL), and the detection limit is as low as 166 ± 10.8 fg mL. It proves that it is completely feasible to amplify sensitivity of sandwich voltammetric immunosensors using polyoxometalate-COF and its catalytic substrate.
鉴于神经元特异性烯醇化酶（NSE）定量的重要性，开发了一种电化学 NSE 免疫传感器。夹心伏安免疫传感器利用乙烯基功能化结晶共价有机框架（COFTAPT-Dva）修饰电极，通过硫醇-烯“点击”反应作为基质负载大量 Ab1。结晶阳离子 EB-COF：Br 用于负载金纳米粒子（AuNPs）和 H3[PMo12O40]（PMo12）作为免疫探针。首先在 EB-COF：Br 上生长约 30nm 的 AuNPs，然后通过离子交换反应将大量电活性 PMo12均匀组装在 AuNPs/EB-COF：Br 上。AuNPs 不仅促进了基于 Au-S 键的 Ab2 结合，而且提高了 Ab2/AuNPs/EB-COF：PMo12 免疫探针的性能。基于负载的丰富 PMo12，可以初步放大夹心电化学免疫传感器的灵敏度。通过使用 PMo12 作为催化剂来催化抗坏血酸，可以实现免疫传感器的二次灵敏度放大。基于差分脉冲伏安法电流变化的夹心伏安免疫传感器的线性范围为 500±36 fg mL - 100±8 ng mL。由于采用了双灵敏度放大策略，灵敏度高达 54.06±3.2 μA cm/lg(cNSE/ng mL)，检测限低至 166±10.8 fg mL。这证明使用多酸-COF 及其催化底物完全可以放大夹心伏安免疫传感器的灵敏度。
