College of Agriculture, South China Agricultural University, Guangzhou, Guangdong 510642, China; Sugarcane Research Laboratory, South China Agricultural University, Guangzhou, Guangdong 510642, China.
College of Agriculture, South China Agricultural University, Guangzhou, Guangdong 510642, China.
Ecotoxicol Environ Saf. 2023 Sep 15;263:115381. doi: 10.1016/j.ecoenv.2023.115381. Epub 2023 Aug 17.
Cadmium (Cd) is a toxic element that endangers crop growth and affects food safety and human health. Therefore, the study of Cd mitigation technology is important. Ultrasonic treatment can improve crop growth and enhance their ability to resist various abiotic stresses. In this study, the effect of ultrasonic treatment on alleviating sugarcane Cd stress was studied in a barrel experiment using sugarcane varieties 'ROC22' and 'LC05-136' as test materials. Sugarcane buds without ultrasonic treatment and with ultrasonic treatment (20-40 kHz mixed frequency ultrasound for 2 min, dry treatment) were planted in soil with Cd contents of 0, 50, 100, 250, and 500 mg·kg. Compared with non-ultrasonic treatment, Ultrasonic treatment significantly increased the activities of antioxidant enzymes in sugarcane, significantly increased the content of osmoregulation substances, significantly reduced the content of superoxide anion (the highest decreases reached 11.55%) and malondialdehyde (the highest decreases reached 20.59%), and significantly increased the expression level of metallothionein (MT)-related genes, with the expression of ScMT1 increased by 8.80-37.49% and the expression of ScMT2-1-5 increased by 1.55-69.33%. In addition, ultrasonic treatment significantly reduced the Cd contents in sugarcane roots, stems, leaves, bagasse, and juice (the highest reduction in Cd content was 49.18%). In general, ultrasonic treatment regulated the metabolism of reactive oxygen species and MT-related gene expression in sugarcane, increased the Cd tolerance of sugarcane, promoted photosynthesis in sugarcane leaves, improved root morphology, enhanced sugarcane growth, and increased cane and sugar yield.
镉（Cd）是一种有毒元素，危害作物生长，影响食品安全和人类健康。因此，研究镉缓解技术很重要。超声处理可以改善作物生长，增强其抵抗各种非生物胁迫的能力。在这项研究中，以甘蔗品种“ROC22”和“LC05-136”为试验材料，在桶实验中研究了超声处理对缓解甘蔗 Cd 胁迫的影响。将未经超声处理和经超声处理（20-40 kHz 混合频率超声 2 分钟，干燥处理）的甘蔗芽种植在 Cd 含量为 0、50、100、250 和 500 mg·kg 的土壤中。与非超声处理相比，超声处理显著提高了甘蔗中抗氧化酶的活性，显著增加了渗透调节物质的含量，显著降低了超氧阴离子（最高降低 11.55%）和丙二醛（最高降低 20.59%）的含量，显著提高了金属硫蛋白（MT）相关基因的表达水平，ScMT1 的表达增加了 8.80-37.49%，ScMT2-1-5 的表达增加了 1.55-69.33%。此外，超声处理显著降低了甘蔗根、茎、叶、蔗渣和汁中的 Cd 含量（Cd 含量的最高降低率为 49.18%）。总的来说，超声处理调节了甘蔗中活性氧和 MT 相关基因表达的代谢，提高了甘蔗对 Cd 的耐受性，促进了甘蔗叶片的光合作用，改善了根系形态，增强了甘蔗的生长，提高了蔗茎和含糖量。
