Diagnostic Research Group, BIONEER Corporation, Daejeon, Republic of Korea.
Department of Biology & Medicinal Science, Pai Chai University, Daejeon, Republic of Korea.
Biochem Biophys Res Commun. 2023 Oct 8;676:213-219. doi: 10.1016/j.bbrc.2023.07.049. Epub 2023 Jul 24.
In this study, bacterial ghosts (BGs) were generated from Weissella koreensis LKS42 (WKorGs) and Pediococcus pentosacues KA94 (PPGs) by chemically inducing lysis using substances such as hydrochloric acid (HCl), sulfuric acid (H2SO4), nitric acid (HNO3), acetic acid (CH3COOH), sodium hydroxide (NaOH), potassium hydroxide (KOH), sodium carbonate (Na2CO3), n-butanol, and C6H8O7. HCl-induced WKorGs and PPGs exhibited complete removal of DNA and displayed transverse membrane dissolution tunnel structures under scanning electron microscopy (SEM). Cell viability assays showed high viability of RAW 264.7 cells exposed to HCl-induced WKorGs and PPGs. Additionally, treatment with HCl-induced WKorGs and PPGs elevated mRNA levels of pro-inflammatory cytokines (IL-1β, IL-6, TNF-α, iNOS) and the anti-inflammatory cytokine IL-10 in RAW 264.7 cells. These findings suggest that HCl-induced WKorGs and PPGs have the potential to be used as inactivated bacterial immunostimulants, highlighting their promising applications in immunization and immunotherapy.
在这项研究中，通过使用盐酸 (HCl)、硫酸 (H2SO4)、硝酸 (HNO3)、乙酸 (CH3COOH)、氢氧化钠 (NaOH)、氢氧化钾 (KOH)、碳酸钠 (Na2CO3)、正丁醇和 C6H8O7 等物质化学诱导裂解，从 Weissella koreensis LKS42 (WKorGs) 和 Pediococcus pentosacues KA94 (PPGs) 中生成了细菌噬菌体 (BGs)。HCl 诱导的 WKorGs 和 PPGs 显示出 DNA 的完全去除，并在扫描电子显微镜 (SEM) 下显示出横向膜溶解隧道结构。细胞活力测定表明，暴露于 HCl 诱导的 WKorGs 和 PPGs 的 RAW 264.7 细胞具有高活力。此外，用 HCl 诱导的 WKorGs 和 PPGs 处理可提高 RAW 264.7 细胞中促炎细胞因子 (IL-1β、IL-6、TNF-α、iNOS) 和抗炎细胞因子 IL-10 的 mRNA 水平。这些发现表明，HCl 诱导的 WKorGs 和 PPGs 有可能被用作失活细菌免疫刺激剂，突出了它们在免疫接种和免疫治疗中的应用前景。
