School of Life Science, Institute of Life Science and Green Development, Hebei University, Baoding 071002, China.
School of Life Science, Institute of Life Science and Green Development, Hebei University, Baoding 071002, China.
J Chromatogr A. 2023 Sep 27;1707:464282. doi: 10.1016/j.chroma.2023.464282. Epub 2023 Aug 6.
In this study, enzyme-deep eutectic solvent-assisted ultrasonic extraction technique (EnDUE) was developed for the efficient dissolution of flavonoids from Artemisiae Argyi Folium. The extraction results of Artemisiae Argyi Folium flavonoids (quercetin, luteolin, and isorhamnetin) were used as indicators to investigate the influencing factors through single factor experiment, Placket-burman design, and Box-behnken design, so as to obtain satisfactory yields. After systematic optimization, the optimal conditions for extraction of the target flavonoids were: Choline chloride/1,4-butanediol with a water content of 25%, cellulase+pectinase with a concentration of 1.6%, solid-liquid ratio of 1/32 g/mL, pH of 4.2, ultrasonic frequency of 80 kHz, ultrasonic power of 160 W, ultrasonic temperature of 40 °C, and ultrasonic time of 25 min, respectively, which derived a total yield of 8.06 ± 0.29 mg/g. Compared with the reference techniques, the proposed EnDUE technique showed significant advantages in the yield and extraction efficiency of flavonoids. In addition, after preliminary purification, the Artemisiae Argyi Folium flavonoids showed good antioxidant activity. Deep eutectic solvent (DES) can degrade the cell wall components and increase the action site of enzyme, and enzyme can promote the penetration of DES into the cell wall matrix, which is mutually beneficial to the dissolution of intracellular components. Therefore, the extraction technique proposed in this work (EnDUE) greatly promotes the dissolution of flavonoids from Artemisiae Argyi Folium, and provides theoretical support for the further application of plant flavonoids.
在这项研究中，开发了酶深共晶溶剂辅助超声提取技术（EnDUE），用于从艾蒿叶中高效溶解黄酮类化合物。以艾蒿叶黄酮类化合物（槲皮素、木樨草素和异鼠李素）的提取率为指标，通过单因素实验、Placket-Burman 设计和 Box-Behnken 设计研究影响因素，以获得满意的产率。经过系统优化，提取目标黄酮类化合物的最佳条件为：氯化胆碱/1,4-丁二醇水含量 25%、纤维素酶+果胶酶浓度 1.6%、固液比 1/32 g/mL、pH 值 4.2、超声频率 80 kHz、超声功率 160 W、超声温度 40°C、超声时间 25 min，总产率为 8.06±0.29 mg/g。与参考技术相比，所提出的 EnDUE 技术在黄酮类化合物的产率和提取效率方面具有显著优势。此外，经过初步纯化，艾蒿叶黄酮类化合物表现出良好的抗氧化活性。深共晶溶剂（DES）可以降解细胞壁成分并增加酶的作用部位，而酶可以促进 DES 渗透到细胞壁基质中，这对细胞内成分的溶解是相互有利的。因此，本工作中提出的提取技术（EnDUE）极大地促进了艾蒿叶中黄酮类化合物的溶解，为植物黄酮类化合物的进一步应用提供了理论支持。
