Liang Long, Zhang Jushan, Duan Hongxia, Li Xuan, Xie Shuanshuan, Wang Changhui
Department of Respiratory Medicine, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, 200072, China.
Department of Oncology, Affiliated Hospital of Nantong University, Nantong, 226006, China.
Cryobiology. 2023 Dec;113:104569. doi: 10.1016/j.cryobiol.2023.104569. Epub 2023 Aug 18.
The aim of this study was to explore the effects of spray cryotherapy (SCT) on cough receptors and airway microenvironment in a canine model of chronic bronchitis. We examined the expression of transient receptor potential vanilloid 1/4 (TRPV1/4) and the neuropeptides substance P (SP) and calcitonin gene-related peptide (CGRP) at the gene and protein levels before and after SCT. In addition, we explored whether TRPV1/4 could regulate inflammatory factors via mediator adenosine triphosphate (ATP). The levels of ATP and cytokines in alveolar lavage fluid and cell supernatant were measured using ELISA. SCT effectively downregulated the expression of TRPV1/4 and SP/CGRP in canine airway tissues with chronic bronchitis and reduced the levels of inflammatory mediators and cytokines that affect cough receptor sensitivity, achieving cough relief. TRPV1/4 - ATP - inflammatory cytokines axis has been demonstrated at the cellular level, which in turn modulate the milieu of the airways and promote the formation of a cough feedback loop. Our study has fully revealed the specific mechanism of SCT in treating cough in a canine model of chronic bronchitis, providing a solid theoretical basis for future clinical treatment.
本研究旨在探讨喷雾冷冻疗法（SCT）对慢性支气管炎犬模型咳嗽感受器及气道微环境的影响。我们检测了SCT前后瞬时受体电位香草酸亚型1/4（TRPV1/4）、神经肽P物质（SP）和降钙素基因相关肽（CGRP）在基因和蛋白水平的表达。此外，我们还探究了TRPV1/4是否可通过介质三磷酸腺苷（ATP）调节炎症因子。采用酶联免疫吸附测定法（ELISA）检测肺泡灌洗液和细胞上清液中ATP和细胞因子的水平。SCT有效下调了慢性支气管炎犬气道组织中TRPV1/4和SP/CGRP的表达，并降低了影响咳嗽感受器敏感性的炎症介质和细胞因子水平，从而缓解了咳嗽。在细胞水平上已证实存在TRPV1/4 - ATP - 炎性细胞因子轴，进而调节气道环境并促进咳嗽反馈环路的形成。我们的研究充分揭示了SCT在慢性支气管炎犬模型中治疗咳嗽的具体机制，为未来的临床治疗提供了坚实的理论基础。
