Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, National Engineering Research Center of Edible Fungi, Shanghai 201403, China.
Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, National Engineering Research Center of Edible Fungi, Shanghai 201403, China.
Int J Biol Macromol. 2023 Nov 1;251:126351. doi: 10.1016/j.ijbiomac.2023.126351. Epub 2023 Aug 18.
This study systematically investigated the therapeutic effects and the corresponding mechanisms of β-D-glucans from Ganoderma lucidum (G. lucidum) with different molecular weights (Ms) on ulcerative colitis (UC). Results showed that three β-d-glucans (GLPS, GLPN and GLPW) from G. lucidum with different Ms exhibited the significant activities on the reduction of typical symptoms of UC by regulating inflammatory cytokine levels, modulating intestinal immunity, improving intestinal microbiota and metabolism of short-chain fatty acids (SCFAs) in the dextran sulfate sodium (DSS)-induced mice model. Among them, the effects of the microwave assisted degraded fraction (GLPW) mainly containing two fractions with smaller M (1.33 × 10 and 3.51 × 10 g/mol) on the regulation of inflammatory factors and SCFAs metabolism were found to be comparable to those of GLPN with medium M (3.49 × 10 g/mol), and superior to those of GLPS with large M (2.42 × 10 g/mol). The effect of GLPW on regulation of intestinal microbiota was even better than that of GLPN. These findings suggested that lowering M by means of physical degradation could improve the anti-inflammatory activities of G. lucidum β-d-glucans. The analysis of anti-inflammatory mechanism also provided a feasible and theoretical basis for potential use of degraded β-d-glucans in the prevention and treatment of UC.
本研究系统地研究了不同分子量（Ms）的灵芝β-D-葡聚糖（G. lucidum）的治疗效果及其相应机制对溃疡性结肠炎（UC）的影响。结果表明，灵芝的三种β-d-葡聚糖（GLPS、GLPN 和 GLPW）具有不同的 Ms，通过调节炎症细胞因子水平、调节肠道免疫、改善肠道微生物群和短链脂肪酸（SCFAs）代谢，对葡聚糖硫酸钠（DSS）诱导的小鼠模型中 UC 的典型症状具有显著的减轻作用。其中，微波辅助降解部分（GLPW）的作用主要包含两个较小分子量的部分（1.33×10 和 3.51×10 g/mol），对炎症因子和 SCFAs 代谢的调节作用与中等分子量（3.49×10 g/mol）的 GLPN 相当，优于高分子量（2.42×10 g/mol）的 GLPS。GLPW 对调节肠道微生物群的作用甚至优于 GLPN。这些发现表明，通过物理降解降低分子量可以提高灵芝β-D-葡聚糖的抗炎活性。抗炎机制的分析也为降解的β-D-葡聚糖在预防和治疗 UC 中的潜在应用提供了可行的理论依据。
