Ma Guan-Hua, Jiang Si-Qi, Liu Li-Ping, Feng Jie, Zhang Jing-Song, Li E-Xian, Li Shu-Hong, Liu Yan-Fang
Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, National Engineering Research Center of Edible Fungi, Shanghai 201403, China.
Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205, China.
Foods. 2024 May 24;13(11):1634. doi: 10.3390/foods13111634.
The adsorption characteristics of β-glucooligosaccharides on activated carbon and the purification were systematically investigated. The maximum adsorption capacity of activated carbon reached 0.419 g/g in the optimal conditions. The adsorption behavior was described to be monolayer, spontaneous, and exothermic based on several models' fitting results. Five fractions with different degrees of polymerization (DPs) and structures of β-glucooligosaccharides were obtained by gradient ethanol elution. 10E mainly contained disaccharides with dp2a (G1→6G) and dp2b (G1→3G). 20E possessed trisaccharides with dp3a (G1→6G1→3G) and dp3b (G1→3G1→3G). 30E mainly consisted of dp3a and dp4a (G1→3G1→3(G1→6)G), dp4b (G1→6G1→3G1→3G), and dp4c (G1→3G1→3G1→3G). In addition to tetrasaccharides, 40E and 50E also contained pentasaccharides and hexasaccharides with β-(1→3)-linked or β-(1→6)-linked glucose residues. All fractions could inhibit the accumulation of intracellular reactive oxygen species (ROS) in HO-induced Caco-2 cells, and they could improve oxidative stress damage by increasing the activity of superoxide dismutase (SOD) and reduced glutathione (GSH), which were related to their DPs and structures. 50E with high DPs showed better anti-oxidative stress activity.
系统研究了β-低聚葡萄糖在活性炭上的吸附特性及纯化过程。在最佳条件下，活性炭的最大吸附量达到0.419 g/g。基于多种模型的拟合结果，吸附行为被描述为单层、自发且放热的。通过梯度乙醇洗脱获得了五个不同聚合度（DPs）和结构的β-低聚葡萄糖级分。10E主要含有二糖dp2a（G1→6G）和dp2b（G1→3G）。20E含有三糖dp3a（G1→6G1→3G）和dp3b（G1→3G1→3G）。30E主要由dp3a和dp4a（G1→3G1→3(G1→6)G）、dp4b（G1→6G1→3G1→3G）和dp4c（G1→3G1→3G1→3G）组成。除四糖外，40E和50E还含有具有β-(1→3)-连接或β-(1→6)-连接葡萄糖残基的五糖和六糖。所有级分均可抑制HO诱导的Caco-2细胞内活性氧（ROS）的积累，且通过提高超氧化物歧化酶（SOD）和还原型谷胱甘肽（GSH）的活性改善氧化应激损伤，这与它们的DPs和结构有关。具有高DPs的50E表现出更好的抗氧化应激活性。
