Marks R M, Penny R
In Vitro Cell Dev Biol. 1986 Jun;22(6):311-6. doi: 10.1007/BF02623403.
Cultures generated from tissues consisting of multiple types of cells are often heterogeneous. Unless the cell type of interest has or can be given some selective growth advantage it may be overgrown by other cells. While developing techniques for the tissue culture of microvascular endothelial cells we evaluated an electrosurgical generator (diathermy) to selectively kill nonendothelial cells. Primary cell cultures were observed at X 100 magnification under phase contrast microscopy and a needle electrode apposed to the cell to be destroyed. A return electrode was constructed by placing a sterile clip in contact with the culture medium. The diathermy power setting controlled the area of lysis. Use of this technique allowed weeding of unwanted cells without damage to endothelial cells, which were able to grow to confluence in pure culture.
由多种类型细胞组成的组织所产生的培养物通常是异质的。除非感兴趣的细胞类型具有或能够获得某种选择性生长优势，否则它可能会被其他细胞过度生长。在开发微血管内皮细胞组织培养技术时，我们评估了一种电外科发生器（透热疗法）来选择性杀死非内皮细胞。在相差显微镜下以100倍放大率观察原代细胞培养物，并将针状电极靠近要破坏的细胞。通过放置一个与培养基接触的无菌夹子来构建一个回路电极。透热疗法的功率设置控制裂解区域。使用该技术可以清除不需要的细胞，而不会损害内皮细胞，内皮细胞能够在纯培养物中生长至汇合。
