纳米基冲洗液对根管牙本质显微硬度的影响：一项离体研究。

The effect of nanobased irrigants on the root canal dentin microhardness: an ex-vivo study.

机构信息

Department of Endodontics, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Fars, Iran.

Endodontist, Department of Endodontics, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.

出版信息

BMC Oral Health. 2023 Aug 19;23(1):581. doi: 10.1186/s12903-023-03298-z.

BACKGROUND

Given the favorable antimicrobial properties of zinc oxide (ZnONPs), standard silver (AgNPs), and imidazolium-based silver (Im-AgNPs) nanoparticles, this study aimed to evaluate their influence on the microhardness of root canal dentin.

METHODS

In this experimental study, 40 mandibular premolars were decoronated at the cementoenamel junction and longitudinally sectioned into halves to create 80 specimens. They were randomly allocated to 5 groups (n = 16) and irrigated with ZnONPs, AgNPs, Im-AgNPs, NaOCl, or normal saline (as the negative control) for 15 min. The Vickers Hardness Number (VHN) was measured on each root canal third before and after being soaked in irrigants. Statistical analysis was performed using paired t-test, one-way ANOVA, and post hoc Tukey's test (α = 0.05).

RESULTS

Im-AgNPs and ZnONPs irrigants improved the microhardness of root dentin, whereas, AgNPs and NaOCl decreased it. ZnONPs yielded the highest VHN at the coronal third (P˂0.001), while the Im-AgNPs provided the highest VHN at the middle and apical thirds (P˂0.001). The AgNPs group showed the lowest VHN at the apical third.

CONCLUSIONS

The irrigants containing Im-AgNPs and ZnONPs significantly enhanced the root dentin microhardness. However, the use of AgNPs resulted in decreased microhardness.

背景

鉴于氧化锌 (ZnONPs)、标准银 (AgNPs) 和咪唑鎓基银 (Im-AgNPs) 纳米粒子具有良好的抗菌性能，本研究旨在评估它们对根管牙本质显微硬度的影响。

方法

在这项实验研究中，将 40 颗下颌前磨牙在牙釉质牙骨质交界处去冠，并纵向切成两半，制成 80 个标本。将它们随机分配到 5 个组（n=16），并用 ZnONPs、AgNPs、Im-AgNPs、次氯酸钠或生理盐水（作为阴性对照）冲洗 15 分钟。在每个根管的第三个部分进行 Vickers 硬度数（VHN）测量，在浸泡在冲洗液之前和之后进行。使用配对 t 检验、单因素方差分析和事后 Tukey 检验（α=0.05）进行统计分析。

结果

Im-AgNPs 和 ZnONPs 冲洗液提高了根管牙本质的显微硬度，而 AgNPs 和次氯酸钠降低了牙本质的显微硬度。ZnONPs 在冠部第三部分产生的 VHN 最高（P<0.001），而 Im-AgNPs 在中部和根尖部提供的 VHN 最高（P<0.001）。AgNPs 组在根尖部的 VHN 最低。