Lindell T D, Nichols B P, Donelson J E, Stellwagen E
Biochim Biophys Acta. 1979 Apr 26;562(2):231-9. doi: 10.1016/0005-2787(79)90169-2.
Individual rapid procedures for the enrichment of Escherichia coli DNA polymerase I and of bacteriophage T4 DNA polymerase free of endonuclease activity are described using Blue dextran-Sepharose chromatography. The blue dye of Blue dextran-Sepharose selectively binds to the deoxynucleoside triphosphate substrate site of the E. coli but not the T4 enzyme indicating that the catalytic sites of these two enzymes which catalyze the same polymerization reaction in vitro are quite distinct.
描述了使用蓝色葡聚糖-琼脂糖凝胶色谱法对不含核酸内切酶活性的大肠杆菌DNA聚合酶I和噬菌体T4 DNA聚合酶进行富集的单独快速程序。蓝色葡聚糖-琼脂糖凝胶的蓝色染料选择性地结合到大肠杆菌酶的脱氧核苷三磷酸底物位点，而不结合T4酶，这表明这两种在体外催化相同聚合反应的酶的催化位点截然不同。
