Smartphone-based electrochemical sensor for cost-effective, rapid and on site detection of chlorogenic acid in herbs using biomass-derived hierarchically porous carbon synthesized by a soft-hard dual template method.

To achieve excellent germplasm resource screening and ensure the quality control of herbal tea raw material, it is important to establish a cost-effective, rapid, and on site quantitative detection method for their bioactive constituents. We developed a smartphone-operated sensor for electrochemical detection of chlorogenic acid (CGA) using hierarchically porous carbon (DSiFPC), synthesized through a soft-hard dual template strategy with tannin acid as a carbon source, silica colloid as a hard template, and Pluronic F127 as a soft template. The DSiFPC modified glassy carbon electrode sensor showed excellent electrocatalytic ability towards CGA, with a wide linear range of 0.03-1 μM and a low limit of detection of 6.2 nM. It was successfully applied for detecting CGA in dried flowers of Lonicera japonica. Furthermore, a portable sensor utilizing a DSiFPC modified screen-printed electrode was employed for on site detection of CGA in fresh Eucommia ulmoides leaves, yielding satisfactory recoveries.