Zhejiang Key Laboratory of Petrochemical Environmental Pollution Control, Zhejiang Ocean University, Zhoushan 316022, China.
College of Marine Science and Technology, Zhejiang Ocean University, Zhoushan 316022, China.
Bioresour Technol. 2023 Nov;387:129696. doi: 10.1016/j.biortech.2023.129696. Epub 2023 Aug 19.
This study focused on the application of salt-tolerant denitrifying bacteria (DBA) in an optimized biofilm process to treat high sulfate-nitrate wastewater from lab-scale to pilot-scale. Lab-scale results demonstrated the salinity, DBA inoculum, supplementary carbon and phosphorus source significantly varied the startup periods at the range of 36-74 d, and the optimum initial start-up conditions were as follows: >0.6 g/L of DBA, 2-4 of C/N ratio, 0.3-0.6 mg/L of phosphorus and a salinity-gradient domestication method. A pilot scale of biofilm technology with DBA was further developed for treating real wastewater from the desulfuration and denitration with both high nitrate (≈200 mg/L) and sulfate (2.7%). The denitrification efficiency reached above 90% after one-month gradient-salinity of 0.5%-2.7%. Mature biofilm had dominant genera Hyphomicrobium (31.80%-61.35%), Methylotenera (0.85%-20.21%) and Thauera (1.42%-8.40%), etc. Notably, the largest genera Hyphomicrobium covered the complete denitrification genes.
本研究专注于耐盐反硝化细菌（DBA）在优化的生物膜工艺中的应用，以处理从实验室规模到中试规模的高硫酸盐-硝酸盐废水。实验室规模的结果表明，盐度、DBA 接种物、补充的碳源和磷源显著改变了启动期，范围在 36-74 天之间，最佳初始启动条件如下：>0.6 g/L 的 DBA、2-4 的 C/N 比、0.3-0.6 mg/L 的磷和盐度梯度驯化方法。进一步开发了 DBA 生物膜技术的中试规模，用于处理脱硫和脱硝过程中同时具有高硝酸盐（≈200 mg/L）和硫酸盐（2.7%）的实际废水。经过一个月的 0.5%-2.7%的梯度盐度后，脱氮效率达到 90%以上。成熟的生物膜具有优势属 Hyphomicrobium（31.80%-61.35%）、Methylotenera（0.85%-20.21%）和 Thauera（1.42%-8.40%）等。值得注意的是，最大的属 Hyphomicrobium 涵盖了完整的反硝化基因。
