Gennaro M L, Novick R P
J Bacteriol. 1986 Oct;168(1):160-6. doi: 10.1128/jb.168.1.160-166.1986.
pT181, a 4.4-kilobase multicopy plasmid of Staphylococcus aureus, encodes a trans-acting initiator protein, RepC, which was rate limiting for replication. Deletions in a 500-base-pair region of the plasmid external to the minimal replicon decreased the ability of the plasmid to compete with a coexisting incompatible plasmid. These deletions, which define a region called cmp (for competition), appeared to affect the interaction of RepC and the plasmid origin of replication. However, in the homoplasmid state the deletions affected neither copy number nor plasmid stability. The Cmp phenotype is orientation independent, and cmp defects could not be complemented in trans.
pT181是金黄色葡萄球菌的一种4.4千碱基对的多拷贝质粒，它编码一种反式作用起始蛋白RepC，该蛋白对复制起限速作用。在最小复制子外部的质粒500碱基对区域中的缺失降低了该质粒与共存的不相容质粒竞争的能力。这些缺失定义了一个称为cmp（竞争）的区域，似乎影响了RepC与质粒复制起点的相互作用。然而，在同质粒状态下，这些缺失既不影响拷贝数也不影响质粒稳定性。Cmp表型与方向无关，并且cmp缺陷不能通过反式互补来弥补。
