Condon Ciarán, Putzer Harald
Institut de Biologie Physico-Chimique, UPR 9073, 13 rue Pierre et Marie Curie, 75005 Paris, France.
Nucleic Acids Res. 2002 Dec 15;30(24):5339-46. doi: 10.1093/nar/gkf691.
Ribonucleases play key, often essential, roles in cellular metabolism. Nineteen ribonuclease activities, from 22 different proteins, have so far been described in bacteria, the majority of them from either Escherichia coli or Bacillus subtilis. Here we examine the phylogenetic distribution of all of these ribonucleases in 50 eubacterial and archaeal species whose genomes have been completely sequenced, with particular emphasis on the endoribonucleases. Although some enzymes are very highly conserved throughout evolution, there appears to be no truly universal ribonuclease. While some organisms, like E.coli, have a large selection of ribonucleases, many with overlapping functions, others seem to have relatively few or have many that remain to be discovered.
核糖核酸酶在细胞代谢中发挥着关键作用，且往往不可或缺。迄今为止，已在细菌中描述了来自22种不同蛋白质的19种核糖核酸酶活性，其中大多数来自大肠杆菌或枯草芽孢杆菌。在此，我们研究了所有这些核糖核酸酶在50种基因组已完全测序的真细菌和古细菌物种中的系统发育分布，尤其着重于内切核糖核酸酶。尽管有些酶在整个进化过程中高度保守，但似乎不存在真正普遍存在的核糖核酸酶。一些生物体，如大肠杆菌，有大量可供选择的核糖核酸酶，其中许多功能重叠，而其他生物体似乎核糖核酸酶相对较少，或者有许多仍有待发现。
