Hase S, Koyama S, Daiyasu H, Takemoto H, Hara S, Kobayashi Y, Kyogoku Y, Ikenaka T
J Biochem. 1986 Jul;100(1):1-10. doi: 10.1093/oxfordjournals.jbchem.a121681.
An asparagine-linked sugar chain of a protease inhibitor from barbados pride (Caesalpinia pulcherrima Sw.) was liberated by hydrazinolysis. After N-acetylation, the reducing end residue of this carbohydrate unit was coupled with 2-aminopyridine and the pyridylamino (PA-) derivative was purified by gel-filtration and reversed-phase HPLC. The structure of the resulting PA-sugar chain was determined mainly by stepwise exoglycosidase digestions and 500 MHz 1H-NMR spectroscopy and proved to be as follows: (formula; see text).
通过肼解作用释放了来自黄蝴蝶（Caesalpinia pulcherrima Sw.）的一种蛋白酶抑制剂的天冬酰胺连接糖链。N-乙酰化后，该碳水化合物单元的还原端残基与2-氨基吡啶偶联，吡啶氨基（PA-）衍生物通过凝胶过滤和反相高效液相色谱法纯化。所得PA-糖链的结构主要通过逐步外切糖苷酶消化和500 MHz 1H-NMR光谱确定，结果如下：（分子式；见正文）。
