Kim D H, Azuma N, Tanaka H, Kanno C
Department of Applied Biochemistry, Utsunomiya University, Japan.
Glycoconj J. 1998 Apr;15(4):361-9. doi: 10.1023/a:1006921818069.
The structures of the N-linked sugar chains in the PAS-6 glycoprotein (PAS-6) from the bovine milk fat globule membrane were determined. The sugar chains were liberated from PAS-6 by hydrazinolysis, and the pyridylaminated sugar chains were separated into a neutral (6N) and two acidic chains (6M and 6D), the acidic sugar chains then being converted to neutral sugar chains (6MN and 6DN). 6N was separated into two neutral fractions (6N13 and 6N5.5), while 6MN and 6DN each gave a single fraction (6MN13 and 6DN13). The structure of 6N5.5, which was the major sugar chain in PAS-6, is proposed to be Man alpha1 --> 6 (Man alpha1 --> 3) Man beta1 --> 4GlcNAc beta1 --> 4GlcNAc-PA; 6N13, 6MN13 and 6DN13 are proposed to be Gal beta1 --> 3Gal beta1 --> 4GlcNAc beta1 --> 2Man alpha1 --> 6 (Gal beta1 --> 3Gal beta1 --> 4GlcNAc beta1 --> 2Man alpha1 --> 3) Man beta1 --> 4GlcNAc beta1 --> 4 (Fuc alpha1 --> 6)GlcNAc-PA; 6M and 6D had 1 or 2 additional NeuAc residues at the non-reducing ends of 6MN13 and 6DN13, respectively.
测定了牛乳脂肪球膜中PAS-6糖蛋白（PAS-6）的N-连接糖链结构。通过肼解从PAS-6中释放出糖链，吡啶氨基化糖链被分离为一种中性糖链（6N）和两种酸性糖链（6M和6D），然后将酸性糖链转化为中性糖链（6MN和6DN）。6N被分离为两个中性组分（6N13和6N5.5），而6MN和6DN各自产生一个单一的组分（6MN13和6DN13）。PAS-6中的主要糖链6N5.5的结构被认为是Manα1→6（Manα1→3）Manβ1→4GlcNAcβ1→4GlcNAc-PA；6N13、6MN13和6DN13被认为是Galβ1→3Galβ1→4GlcNAcβ1→2Manα1→6（Galβ1→3Galβ1→4GlcNAcβ1→2Manα1→3）Manβ1→4GlcNAcβ1→4（Fucα1→6）GlcNAc-PA；6M和6D分别在6MN13和6DN13的非还原末端有1个或2个额外的NeuAc残基。
