Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Mansoura University, Mansoura, 35516, Egypt.
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Delta University for Science and Technology, International Coastal Road, Gamasa, 11152, Egypt.
Sci Rep. 2023 Aug 20;13(1):13549. doi: 10.1038/s41598-023-40559-x.
Orphenadrine (ORP), dimenhydrinate (DMN), and cinnarizine (CNN) were investigated using green-sensitive spectrofluorometric methods. Method, I used for determination of DMN in 0.1 M hydrochloric acid (HCl) and 1.0% sodium dodecyl sulphate (SDS) at 286 nm after λ 222 nm, while for determination of ORP in 1.0% w/v SDS involves measuring the fluorescence at 285 nm after λ 220 nm. For DMN and ORP, the detection and quantitation limits were 2.99 and 4.71 and 9.08 and 14.29 ng/mL, respectively. The ranges of DMN and ORP were 0.10-1.0 and 0.04-0.5 µg/mL, respectively, in micellar aqueous solution. Method II, the derivative intensities of DMN and CNN were measured at a fixed of different wavelength between the excitation and the emission wavelengths (Δλ) = 60 nm at 282 and 322 nm, at the zero crossing of each other, respectively. The detection and quantitation limits for DMN and CNN were 1.77 and 0.88 ng/mL and 5.36 and 2.65 ng/mL, correspondingly, through the entire range of 0.1-1.0 µg/mL for DMN and CNN. The linearity was perfectly determined through the higher values of the correlation coefficient ranging from 0.9997 to 0.9999 for both direct and synchronous methods. The precision of the proposed methods was also confirmed via the lower values of the standard deviation which ranged from 0.39 to 1.11. The technique was expanded to analyze this mixture in combined tablets and laboratory-prepared mixtures. The method validation was done depending on the international conference on harmonization (ICH) recommendations. An analysis of the statistical data revealed a high agreement between the proposed data and the comparison methodology. Three different assessment methods demonstrated the greenness of the technique.
奥芬那君（ORP）、茶苯海明（DMN）和桂利嗪（CNN）被研究采用了绿色敏感的分光荧光法。方法 I 用于在 0.1 M 盐酸（HCl）和 1.0%十二烷基硫酸钠（SDS）中测定 DMN，在 286nm 后λ 222nm，而在 1.0% w/v SDS 中测定 ORP 涉及在 285nm 后λ 220nm 测量荧光。对于 DMN 和 ORP，检测限和定量限分别为 2.99 和 4.71ng/mL 以及 9.08 和 14.29ng/mL。DMN 和 ORP 的范围分别为 0.10-1.0 和 0.04-0.5μg/mL，在胶束水溶液中。方法 II，DMN 和 CNN 的导数强度在激发和发射波长之间的不同波长（Δλ）= 60nm 处测量，分别在 282nm 和 322nm 处，在彼此的零交点处。DMN 和 CNN 的检测限和定量限分别为 1.77 和 0.88ng/mL 和 5.36 和 2.65ng/mL，分别对应于 0.1-1.0μg/mL 范围内的 DMN 和 CNN。直接和同步方法的相关系数值均高于 0.9997 到 0.9999，通过线性度得到了完美的确定。通过标准偏差值的降低，证明了所提出方法的精度，标准偏差值范围从 0.39 到 1.11。该技术被扩展用于分析混合片剂和实验室制备的混合物。方法验证取决于国际协调会议（ICH）的建议。统计数据的分析表明，所提出的数据与比较方法高度一致。三种不同的评估方法证明了该技术的绿色性。
