Department of Botany, Aligarh Muslim University, Aligarh, 202002, India.
Biotechnology and Molecular Biology Division, CSIR-National Botanical Research Institute, Lucknow, 226001, India.
Environ Sci Pollut Res Int. 2023 Dec;30(56):118246-118262. doi: 10.1007/s11356-023-29204-2. Epub 2023 Aug 21.
Lead (Pb) is one of the most toxic elements on earth. The harmful effects of Pb at higher concentrations were seen on plant vegetation because plants are directly exposed towards it. Indian mustard, a well-known hyperaccumulator plant is the most promising crop for the environment, engaged in a variety of scenarios for ecological cleanup. In the present study, we used ethylene diamine tetraacetic acid (EDTA), a chelating agent that is of remarkable efficiency. The pot experiments were conducted in soil pretreated with 1000 mgkg Pb with different concentrations of EDTA (2-10 mmol). All the growth parameters were reduced significantly in the plants treated with Pb and EDTA, however, a non-significant effect was observed in 5 mmol EDTA compared to Pb alone treatment. Photosynthetic pigments yield, nitrate reductase activity and NPK content were affected negatively; in contrast, superoxide dismutase and catalase activity was increased in Pb and Pb+EDTA treated in both the varieties. The Pb accumulation was elevated significantly by the augmentation of 5 mmol EDTA in both varieties. Accumulation of Pb in the shoot was higher in PM 25 than in P. Vijay, whereas root Pb accumulation showed the opposite, i.e., more Pb in roots of P. Vijay than PM 25. Moreover, The Pb accumulation per plant was observed more in P. Vijay as compared to PM 25. Hence, the present study implies that the augmentation of Pb-polluted soil with EDTA works well while dealing with B. juncea assisted phytoremediation and P. Vijay to be a stronger variety than PM 25. Further, 5 mmol of EDTA was optimum for phytoremediation of the soil polluted with up to 1000 mg Pb kg soil.
铅（Pb）是地球上毒性最大的元素之一。由于植物直接暴露在其中，高浓度的 Pb 对植物植被产生了有害影响。芥菜，一种众所周知的超富集植物，是最有前途的环境作物，在各种生态清理场景中都有应用。在本研究中，我们使用了乙二胺四乙酸（EDTA），这是一种效率显著的螯合剂。在预处理过的土壤中进行了盆栽实验，土壤中 Pb 含量为 1000mgkg，EDTA 浓度分别为 2-10mmol。用 Pb 和 EDTA 处理的植物的所有生长参数均显著降低，但与单独用 Pb 处理相比，在 5mmol EDTA 处理时，效果并不显著。光合色素产量、硝酸还原酶活性和 NPK 含量均呈负相关；相反，超氧化物歧化酶和过氧化氢酶活性在两种品种的 Pb 和 Pb+EDTA 处理中均增加。两种品种的 5mmol EDTA 均可显著提高 Pb 的积累。在 PM 25 中，Pb 在地上部分的积累量高于 P. Vijay，但根部分的 Pb 积累则相反，即 P. Vijay 根中的 Pb 含量高于 PM 25。此外，与 PM 25 相比，P. Vijay 每株植物的 Pb 积累量更多。因此，本研究表明，在处理芥菜辅助植物修复过程中，用 EDTA 处理 Pb 污染土壤效果良好，而 P. Vijay 比 PM 25 更具优势。此外，在 1000mg Pb kg 土壤污染土壤的植物修复中，5mmol EDTA 是最适宜的。
