Faculty of Pharmacy, Cyprus International University, Haspolat/Nicosia, North Cyprus, Via, Mersin 10 Turkey/Department of Pharmacognosy and Ethnopharmacy, Faculty of Pharmaceutical Sciences, Usmanu Danfodiyo University Sokoto, Sokoto State, Nigeria.
Department of Medical Laboratory Sciences, Bingham University, Karu, Nasarawa state, Nigeria.
Pak J Pharm Sci. 2023 Jul;36(4):1233-1239.
Parinari curatellifolia is mostly employed in the treatments of leukemia, anemia and malaria. The study was to determine the hematological, biochemical and histopathological effects of methanol stem bark extract of Parinari curatellifolia (PCME) on liver and kidney of adult female Wistar rats. The oral acute (Lorke's method) and sub-chronic toxicity of PCME were evaluate. Adult female Wistar rats were grouped into group I (n=6), normal control (5mL/kg of distilled water) and groups II-IV (100, 200 and 400mg/kg/day of PCME, n=6 each) for 30 days. On 31st day, biochemical, hematological and histopathological parameters were assessed. The LD was found greater than 5000mg/kg. In hematological parameters, RBC showed an increase in the treatment groups, however, the increment was not significant. HCT, PLT, MCH and MCHC levels were significantly increased (p<0.05) while WBC levels in all PCME groups were reduced (p<0.05). Amongst the liver biochemical parameters, only the ALP activity was significantly (p<0.05) raised. In kidney biochemical parameters, serum potassium and chloride were significantly (p<0.05) reduced. Histopathological findings on the liver showed mild infiltrating leukocytes, vascular congestion and piece meal necrosis compared to the normal anatomic features while that of the kidney appeared normal. In conclusion, PCME may be slightly toxic to the liver on repeated administration.
皱果巴戟天主要用于治疗白血病、贫血和疟疾。本研究旨在确定皱果巴戟天甲醇树皮提取物（PCME）对成年雌性 Wistar 大鼠肝脏和肾脏的血液学、生化和组织病理学影响。评估了 PCME 的口服急性（Lorke 法）和亚慢性毒性。成年雌性 Wistar 大鼠分为 I 组（n=6），正常对照组（5mL/kg 蒸馏水）和 II-IV 组（100、200 和 400mg/kg/天的 PCME，每组 n=6），共 30 天。第 31 天，评估生化、血液学和组织病理学参数。LD 大于 5000mg/kg。在血液学参数方面，RBC 在治疗组中增加，但增加不显著。HCT、PLT、MCH 和 MCHC 水平显著升高（p<0.05），而所有 PCME 组的 WBC 水平均降低（p<0.05）。在肝脏生化参数中，只有 ALP 活性显著升高（p<0.05）。在肾脏生化参数中，血清钾和氯显著降低（p<0.05）。肝脏的组织病理学发现与正常解剖特征相比，有轻度浸润性白细胞、血管充血和碎片状坏死，而肾脏则正常。总之，PCME 重复给药可能对肝脏有轻微毒性。
