Sam-Ang Pornpat, Phanumartwiwath Anuchit, Liana Desy, Sureram Sanya, Hongmanee Poonpilas, Kittakoop Prasat
Department of Chemistry, Faculty of Science and Technology, Pibulsongkram Rajabhat University, Phitsanulok 65000, Thailand.
College of Public Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
ACS Omega. 2023 Aug 1;8(32):29615-29624. doi: 10.1021/acsomega.3c03656. eCollection 2023 Aug 15.
is a medicinal plant that has been traditionally used in various therapeutic applications. All parts of including fruits, leaves, stems, roots, and flowers contain various biologically active phytochemicals. This study aimed to evaluate the antitubercular, antibacterial, and antioxidant activities of root extracts and spectroscopically analyze the bioactive metabolites. root extracts were prepared via maceration. The minimum inhibitory concentration (MIC) for antitubercular activity, the inhibition zone for antibacterial activity, and the antioxidant activities in terms of half-maximal inhibitory concentration (IC) values were determined. H-NMR, RP-HPLC, and UHPLC-QQQ-MS analyses were performed to evaluate the secondary metabolites. The results showed that the dichloromethane root extract exhibited relatively good inhibition of with an MIC value of 50 μg/mL. All extracts were mostly active against five tested bacterial strains. The ethanolic and dichloromethane root extracts showed the highest antioxidant power against DPPH (IC = 0.82 mg/mL) and NO (IC = 0.64 mg/mL) radicals, respectively. The H-NMR-based screening of the secondary metabolites of all root extracts confirmed the presence of triterpenes, steroids, phenolics, flavonoids, tannins, and anthraquinones as major bioactive components. Alizarin and scopoletin were detected in the extracts via UHPLC-QQQ-MS, and the alizarin (0.552-3.227 g/100 g dry weight) and scopoletin (0.092-0.554 g/100 g dry weight) contents were quantified via RP-HPLC. The antimicrobial and antioxidant activities of root extracts and the identification of the main bioactive ingredients are the initial studies that can be beneficial for further in vivo studies and biomedical applications of its bioactive compounds.
是一种传统上用于各种治疗应用的药用植物。其所有部位，包括果实、叶子、茎、根和花，都含有各种具有生物活性的植物化学物质。本研究旨在评估其根提取物的抗结核、抗菌和抗氧化活性，并通过光谱分析生物活性代谢物。其根提取物通过浸渍法制备。测定了抗结核活性的最低抑菌浓度（MIC）、抗菌活性的抑菌圈以及以半数最大抑制浓度（IC）值表示的抗氧化活性。进行了¹H-NMR、RP-HPLC和UHPLC-QQQ-MS分析以评估次生代谢物。结果表明，二氯甲烷根提取物对[具体结核杆菌名称未给出]表现出相对较好的抑制作用，MIC值为50μg/mL。所有提取物对五种受试细菌菌株大多具有活性。乙醇根提取物和二氯甲烷根提取物分别对DPPH（IC = 0.82mg/mL）和NO（IC = 0.64mg/mL）自由基表现出最高的抗氧化能力。基于¹H-NMR对所有其根提取物次生代谢物的筛选证实，三萜类、甾体类、酚类、黄酮类、单宁类和蒽醌类作为主要生物活性成分存在。通过UHPLC-QQQ-MS在提取物中检测到了茜素和东莨菪素，并通过RP-HPLC对茜素（0.552 - 3.227g/100g干重）和东莨菪素（0.092 - 0.554g/100g干重）含量进行了定量。其根提取物的抗菌和抗氧化活性以及主要生物活性成分的鉴定是初步研究，可能有利于其生物活性化合物的进一步体内研究和生物医学应用。
