Research Chair in Laser Diagnosis of Cancers, Department of Physics and Astronomy, College of Science, King Saud University, P.O. Box, 2455, Riyadh, 11451, Saudi Arabia.
Department of Chemistry, Tiruvallur University, Serkkadu, Vellore, 632 115, Tamil Nadu, India.
Chemosphere. 2023 May;323:138179. doi: 10.1016/j.chemosphere.2023.138179. Epub 2023 Feb 25.
In this study, aqueous and methanol extracts of Morinda coreia (MC) leaves were tested for antioxidant and antibacterial activity under in vitro conditions. Phytochemical analysis using UPLC-ESI-MS revealed the presence of phenolics, flavonoids, alkaloids, glycosides, amino acids, proteins, saponins, and tannins. Under in vitro conditions, antioxidant test using DPPH, ABTS, and reducing power demonstrated that the plant leaves play a crucial role in antioxidant activity compared to the commercial antioxidant butylated hydroxytoluene (BHT). The ABTS and DPPH free radical scavenging activities showed that the IC values of the M. coreia methanol extract were 26.35 μg/mL and 200.23 μg/mL, respectively. The methanol extract of M. coreia contained higher levels of total phenols and flavonoids and higher free radical scavenging capacity than the aqueous extract. FTIR analysis of the methanol extract showed a substantial number of phenols in the functional groups of M. coreia leaves. The well diffusion assay using the methanolic extract of M. coreia (200 μg/mL) leaves showed antibacterial activity against Pseudomonas aeruginosa (19 ± 0.85 mm), Proteus sp. (20 ± 0.97 mm), Streptococcus sp. (21 ± 1.29 mm), and Enterobacter sp. (17 ± 0.2 mm). Thus, the present study revealed that the antibacterial and antioxidant activity of M. coreia leaf extract was due to the presence of 18 unknown and 15 primary known polyphenols.
在这项研究中，对辣木（Morinda coreia）叶的水提物和甲醇提取物进行了体外抗氧化和抗菌活性测试。使用 UPLC-ESI-MS 的植物化学分析显示存在酚类、类黄酮、生物碱、糖苷、氨基酸、蛋白质、皂苷和单宁。在体外条件下，使用 DPPH、ABTS 和还原力的抗氧化测试表明，与商业抗氧化剂丁基羟基甲苯 (BHT) 相比，植物叶片在抗氧化活性中起着至关重要的作用。ABTS 和 DPPH 自由基清除活性表明，辣木叶甲醇提取物的 IC 值分别为 26.35μg/mL 和 200.23μg/mL。辣木叶甲醇提取物中总酚类和类黄酮含量较高，自由基清除能力较强。甲醇提取物的 FTIR 分析显示辣木叶的功能基团中存在大量酚类物质。使用辣木叶甲醇提取物（200μg/mL）的良好扩散测定法显示对铜绿假单胞菌（19±0.85mm）、变形杆菌（20±0.97mm）、链球菌（21±1.29mm）和肠杆菌（17±0.2mm）具有抗菌活性。因此，本研究表明，辣木叶提取物的抗菌和抗氧化活性归因于 18 种未知和 15 种主要已知多酚的存在。
