Liu Chengcheng, Yu Jingsheng, Ying Jizhe, Zhang Kai, Hu Zhigang, Liu Zhixiang, Chen Shilin
College of Pharmacy, Hubei University of Chinese Medicine, Wuhan, China.
Institute of Herbgenomics, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
Front Plant Sci. 2023 Aug 4;14:1223720. doi: 10.3389/fpls.2023.1223720. eCollection 2023.
(FU) is a renowned herb in China that requires strict growth conditions in its cultivation process. During this process, the soil microorganisms and their metabolites may directly affect the growth and development of FU, for example, the pathogen infection and sipeimine production. However, few systematic studies have reported the changes in the microbiome and metabolites during FU cultivation thus far. In this work, we simultaneously used metagenomics and metabolomics technology to monitor the changes in microbial communities and metabolites in the rhizosphere of FU during its cultivation for one, two, and three years. Moreover, the interaction between microorganisms and metabolites was investigated by co-occurrence network analysis. The results showed that the microbial composition between the three cultivation-year groups was significantly different (2020-2022). The dominant genera changed from and in CC1 to and in CC3. The relative abundances of beneficial microorganisms decreased, while the relative abundances of harmful microorganisms showed an increasing trend. The metabolomics results showed that significant changes of the of metabolite composition were observed in the rhizosphere soil, and the relative abundances of some beneficial metabolites showed a decreasing trend. In this study, we discussed the changes in the microbiome and metabolites during the three-year cultivation of FU and revealed the relationship between microorganisms and metabolites. This work provides a reference for the efficient and sustainable cultivation of FU.
附子是中国一种著名的草药，其种植过程需要严格的生长条件。在此过程中，土壤微生物及其代谢产物可能直接影响附子的生长发育，例如病原体感染和次乌头碱的产生。然而，迄今为止，很少有系统研究报道附子种植过程中微生物群落和代谢产物的变化。在这项工作中，我们同时使用宏基因组学和代谢组学技术来监测附子种植1年、2年和3年期间根际微生物群落和代谢产物的变化。此外，通过共现网络分析研究了微生物与代谢产物之间的相互作用。结果表明，三个种植年份组（2020 - 2022年）之间的微生物组成存在显著差异。优势属从CC1中的 和 变为CC3中的 和 。有益微生物的相对丰度下降，而有害微生物的相对丰度呈上升趋势。代谢组学结果表明，根际土壤中代谢产物组成发生了显著变化，一些有益代谢产物的相对丰度呈下降趋势。在本研究中，我们讨论了附子三年种植过程中微生物群落和代谢产物的变化，并揭示了微生物与代谢产物之间的关系。这项工作为附子的高效可持续种植提供了参考。
