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Assembly and analysis of the mitochondrial genome of .

作者信息

Sun Zhihao, Wu Ya, Fan Pengyu, Guo Dengli, Zhang Sanyin, Song Chi

机构信息

Institute of Herbgenomics, Chengdu University of Traditional Chinese Medicine, Chengdu, China.

Wuhan Benagen Technology Co., Ltd, Wuhan, Hubei, China.

出版信息

Front Plant Sci. 2023 Aug 2;14:1237822. doi: 10.3389/fpls.2023.1237822. eCollection 2023.

Abstract

(Lamiaceae) is widely distributed in Eurasia. Former studies have demonstrated that has a wide range of pharmacological effects. Nevertheless, no complete mitochondrial genome has been reported, which limits further understanding of the biology of . Here, we assembled the first complete mitochondrial genome of using a hybrid assembly strategy based on sequencing data from both Nanopore and Illumina platforms. Then, the mitochondrial genome of was analyzed comprehensively in terms of gene content, codon preference, intercellular gene transfer, phylogeny, and RNA editing. The mitochondrial genome of has two circular structures. It has a total length of 297, 777 bp, a GC content of 43.92%, and 29 unique protein-coding genes (PCGs). There are 76 simple sequence repeats (SSRs) in the mitochondrial genome, of which tetrameric accounts for a large percentage (43.4%). A comparative analysis between the mitochondrial and chloroplast genomes revealed that 36 homologous fragments exist in them, with a total length of 28, 895 bp. The phylogenetic analysis showed that belongs to the Lamiales family Lamiaceae and is closely related to . In addition, the mitochondrial genome sequences of seven species of Lamiaceae are unconservative in their alignments and undergo frequent genome reorganization. This work reports for the first time the complete mitochondrial genome of , which provides useful genetic information for further studies.

摘要

唇形科在欧亚大陆广泛分布。先前的研究表明,其具有广泛的药理作用。然而,尚未有完整的线粒体基因组被报道,这限制了对其生物学特性的进一步了解。在此,我们基于来自纳米孔和Illumina平台的测序数据,采用混合组装策略,组装出了该植物的首个完整线粒体基因组。然后,从基因内容、密码子偏好、细胞间基因转移、系统发育和RNA编辑等方面对该植物的线粒体基因组进行了全面分析。该植物的线粒体基因组有两个环状结构。其全长297,777 bp,GC含量为43.92%,有29个独特的蛋白质编码基因(PCGs)。线粒体基因组中有76个简单序列重复(SSRs),其中四聚体占比很大(43.4%)。线粒体基因组与叶绿体基因组的比较分析表明,它们之间存在36个同源片段,总长度为28,895 bp。系统发育分析表明,该植物属于唇形目唇形科,且与[具体物种]关系密切。此外,唇形科七种植物的线粒体基因组序列在比对中不保守,且频繁发生基因组重排。这项工作首次报道了该植物的完整线粒体基因组,为进一步的[具体植物名称]研究提供了有用的遗传信息。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/107c/10433383/76ed1bd96e4d/fpls-14-1237822-g001.jpg

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