Schöneshöfer M, Kage A, Eisenschmid B, Heilmann P, Dhar T K, Weber B
J Chromatogr. 1986 Aug 2;380(2):267-74. doi: 10.1016/s0378-4347(00)83655-5.
A fully automated method for the simultaneous assessment of cortisol, cortisone and their 20-dihydro isomers in human urine is described. On-line sample enrichment, prepurification, focusing and injection are combined with automated high-performance liquid chromatographic separation and quantification. Losses of steroids throughout the total procedure are negligible. Thus, external calibration is feasible for quantification. Coefficients of variation range between 8.7 and 17.0% for inter-assay variability and between 1.3 and 5.2% for intra-assay variability. Assay sensitivity is 15 nmol/l. In normal students, the medians of the relative excretion rates of free 20 alpha-dihydrocortisol, 20 alpha-dihydrocortisone, 20 beta-dihydrocortisol and 20 beta-dihydrocortisone were 10.9, 6.1, 7.7 and 4.4 mumol/mol creatinine. The fully automated feature renders the present method well suited for routine diagnosis of hypercorticoidism.
本文描述了一种用于同时测定人尿中皮质醇、可的松及其20-二氢异构体的全自动方法。在线样品富集、预纯化、聚焦和进样与自动高效液相色谱分离和定量相结合。整个过程中类固醇的损失可忽略不计。因此，外部校准定量是可行的。批间变异系数在8.7%至17.0%之间，批内变异系数在1.3%至5.2%之间。测定灵敏度为15 nmol/l。在正常学生中，游离20α-二氢皮质醇、20α-二氢可的松、20β-二氢皮质醇和20β-二氢可的松的相对排泄率中位数分别为10.9、6.1、7.7和4.4 μmol/mol肌酐。全自动特性使本方法非常适合用于皮质醇增多症的常规诊断。
