National Clinical Research Center for Infectious Disease, Shenzhen Third People's Hospital, Shenzhen, Guangdong, China.
Center for Cellular and Molecular Diagnostics, Tulane University School of Medicine, New Orleans, LA, United States.
Front Immunol. 2023 Aug 3;14:1172035. doi: 10.3389/fimmu.2023.1172035. eCollection 2023.
Tuberculosis (TB) remains a major underdiagnosed public health threat worldwide, being responsible for more than 10 million cases and one million deaths annually. TB diagnosis has become more rapid with the development and adoption of molecular tests, but remains challenging with traditional TB diagnosis, but there has not been a critical review of this area. Here, we systematically review these approaches to assess their diagnostic potential and issues with the development and clinical evaluation of proposed CRISPR-based TB assays. Based on these observations, we propose constructive suggestions to improve sample pretreatment, method development, clinical validation, and accessibility of these assays to streamline future assay development and validation studies.
结核病（TB）仍然是全球未被充分诊断的主要公共卫生威胁，每年导致超过 1000 万例病例和 100 万人死亡。随着分子检测的发展和采用，结核病诊断变得更加迅速，但传统的结核病诊断仍然具有挑战性，但该领域尚未进行批判性审查。在这里，我们系统地回顾了这些方法，以评估它们的诊断潜力以及基于 CRISPR 的 TB 检测方法的开发和临床评估中存在的问题。基于这些观察结果，我们提出了建设性的建议，以改善这些检测方法的样本预处理、方法开发、临床验证和可及性，以简化未来的检测方法开发和验证研究。
