van Gulpen C, Brokerhof A W, van der Kaay M, Tjaden U R, Mattie H
J Chromatogr. 1986 Sep 5;381(2):365-72. doi: 10.1016/s0378-4347(00)83602-6.
A method for the determination of benzylpenicillin and probenecid concentrations in human body fluids using ion-pair reversed-phase chromatography with UV detection has been developed. For plasma samples two extraction techniques were investigated. Precipitation of the plasma proteins with acetonitrile followed by liquid-liquid extraction offered the best results. The limits of detection were 0.5 microgram/ml for benzylpenicillin and 0.25 microgram/ml for probenecid, which offer sufficient sensitivity for application in pharmacokinetic experiments.
已开发出一种使用离子对反相色谱结合紫外检测法测定人体体液中苄青霉素和丙磺舒浓度的方法。对于血浆样品，研究了两种提取技术。用乙腈沉淀血浆蛋白后进行液液萃取效果最佳。苄青霉素的检测限为0.5微克/毫升，丙磺舒的检测限为0.25微克/毫升，这为药代动力学实验提供了足够的灵敏度。
