Li Ningning, Zhang Xiuzhi, Cui Yanan, Wu Hui, Yu Yue, Yu Shanfa
Department of Pathology, Henan Medical College, Zhengzhou, Henan, China.
Department of Occupational and Environmental Health, College of Public Health, Zhengzhou University, Zhengzhou, Henan, China.
Front Microbiol. 2023 Aug 4;14:1229407. doi: 10.3389/fmicb.2023.1229407. eCollection 2023.
Noise exposure could lead to hearing loss and disorders of various organs. Recent studies have reported the close relations of environmental noise exposure to the metabolomics dysregulations and gut microbiota disturbance in the exposers. However, the associations between gut microbial homeostasis and the body metabolism during noise-induced hearing loss (NIHL) were unclear. To get a full understanding of their synergy in noise-associated diseases, it is essential to uncover their impacts and associations under exposure conditions.
With ten male rats with background noise exposure (≤ 40 dB) as controls (Ctr group), 20 age- and weight-matched male rats were exposed to 95 dB Sound pressure level (SPL) (LN group, = 10) or 105 dB SPL noise (HN group, = 10) for 30 days with 4 h/d. The auditory brainstem response (ABR) of the rats and their serum biochemical parameters were detected to investigate their hearing status and the potential effects of noise exposure on other organs. Metabolomics (UPLC/Q-TOF-MS) and microbiome (16S rDNA gene sequencing) analyses were performed on samples from the rats. Multivariate analyses and functional enrichments were applied to identify the dysregulated metabolites and gut microbes as well as their associated pathways. Pearson correlation analysis was performed to investigate the associations of the dysregulations of microbiota and the metabolites.
NIHL rat models were constructed. Many biochemical parameters were altered by noise exposure. The gut microbiota constitution and serum metabolic profiles of the noise-exposed rats were also dysregulated. Through metabolomics analysis, 34 and 36 differential metabolites as well as their associated pathways were identified in LN and HN groups, respectively. Comparing with the control rats, six and 14 florae were shown to be significantly dysregulated in the LN group and HN group, respectively. Further association analysis showed significant correlations between differential metabolites and differential microbiota.
There were cochlea injuries and abnormalities of biochemical parameters in the rats with NIHL. Noise exposure could also disrupt the metabolic profiles and the homeostatic balance of gut microbes of the host as well as their correlations. The dysregulated metabolites and microbiota might provide new clues for prevention of noise-related disorders.
噪声暴露可导致听力损失和各器官功能紊乱。近期研究报道了环境噪声暴露与暴露者代谢组学失调及肠道微生物群紊乱之间的密切关系。然而，噪声性听力损失（NIHL）期间肠道微生物稳态与机体代谢之间的关联尚不清楚。为全面了解它们在噪声相关疾病中的协同作用，有必要揭示它们在暴露条件下的影响和关联。
以10只暴露于背景噪声（≤40dB）的雄性大鼠作为对照组（Ctr组），将20只年龄和体重匹配的雄性大鼠暴露于95dB声压级（SPL）（LN组，n = 10）或105dB SPL噪声（HN组，n = 10）中，每天暴露4小时，持续30天。检测大鼠的听觉脑干反应（ABR）及其血清生化参数，以研究它们的听力状况以及噪声暴露对其他器官的潜在影响。对大鼠样本进行代谢组学（UPLC/Q-TOF-MS）和微生物组（16S rDNA基因测序）分析。应用多变量分析和功能富集来鉴定失调的代谢物和肠道微生物及其相关途径。进行Pearson相关性分析以研究微生物群失调与代谢物之间的关联。
构建了NIHL大鼠模型。噪声暴露改变了许多生化参数。噪声暴露大鼠的肠道微生物群组成和血清代谢谱也发生了失调。通过代谢组学分析，在LN组和HN组中分别鉴定出34种和36种差异代谢物及其相关途径。与对照大鼠相比，LN组和HN组分别有6种和14种菌群显示出明显失调。进一步的关联分析表明差异代谢物与差异微生物群之间存在显著相关性。
NIHL大鼠存在耳蜗损伤和生化参数异常。噪声暴露还可能破坏宿主的代谢谱和肠道微生物的稳态平衡及其相关性。失调的代谢物和微生物群可能为预防噪声相关疾病提供新线索。
