School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China.
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2022 Nov 15;1211:123459. doi: 10.1016/j.jchromb.2022.123459. Epub 2022 Sep 13.
Semen Euphorbiae (SE) is a toxic traditional Chinese medicine made from the dry or mature seed of Euphorbia lathyris L. Research demonstrates that the toxic side-effects from eating SE are associated with intestinal disturbance. By processing to produce Semen Euphorbiae Pulveratum (SEP), the toxicity is reduced, and diarrhea is attenuated. However, there are minimal studies on the differential effects between SE and SEP on microbiota and fecal metabolites. In this study, 16S rDNA sequencing and UPLC-Q-TOF/MS were interpreted with PCA and OPLS-DA multivariate analysis to understand the effect of SE and SEP on the gut microbiota and fecal metabolic phenotype in rats. Compared to the blank control group, the results showed that both SE and SEP were associated with increased microbes from the phylum Firmicutes and decreased Bacteroidetes, but the change was not as strong in the SEP administration group. Meanwhile, the fecal metabolism of rats also changed significantly, since 17 additional metabolites were detected in both groups, including amino acid metabolites, bacterial metabolites, and lipid metabolites. Our results indicate that the SEP administration group may reduce toxicity by differentially influencing intestinal metabolites and flora.
大戟（SE）是一种由大戟属植物干燥或成熟种子制成的有毒中药。研究表明，食用 SE 的毒副作用与肠道紊乱有关。通过加工生产大戟粉（SEP），可以降低毒性，减轻腹泻。然而，关于 SE 和 SEP 对微生物群和粪便代谢物的差异影响的研究很少。在这项研究中，采用 16S rDNA 测序和 UPLC-Q-TOF/MS 进行分析，并进行 PCA 和 OPLS-DA 多变量分析，以了解 SE 和 SEP 对大鼠肠道微生物群和粪便代谢表型的影响。与空白对照组相比，结果表明，SE 和 SEP 均与厚壁菌门的微生物增加和拟杆菌门的减少有关，但 SEP 给药组的变化并不那么强烈。同时，大鼠的粪便代谢也发生了显著变化，因为两组都检测到了 17 种额外的代谢物，包括氨基酸代谢物、细菌代谢物和脂质代谢物。我们的研究结果表明，SEP 给药组可能通过影响肠道代谢物和菌群来降低毒性。
