香蕉病抑土壤促使对香蕉枯萎病形成防御。

Banana disease-suppressive soil drives assembled to defense Fusarium wilt of banana.

作者信息

Fan Huacai, He Ping, Xu Shengtao, Li Shu, Wang Yongfen, Zhang Wenlong, Li Xundong, Shang Hui, Zeng Li, Zheng Si-Jun

机构信息

Yunnan Key Laboratory of Green and Control of Agricultural Transboundary Pests, Agricultural Environment and Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming, China.

State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Ministry of Education Key Laboratory of Agriculture Biodiversity for Plant Disease Management, College of Plant Protection, Yunnan Agricultural University, Kunming, China.

出版信息

Front Microbiol. 2023 Aug 3;14:1211301. doi: 10.3389/fmicb.2023.1211301. eCollection 2023.

Fusarium wilt of banana (FWB) caused by f. sp. tropical race 4 ( TR4), poses a serious problem for sustainable banana production. Biological control is one of the effective measures to control this destructive disease. High-throughput sequencing of soil microorganisms could significantly improve the efficiency and accuracy of biocontrol strain screening. In this study, the soil microbial diversity of six main banana-producing areas in Yunnan was sequenced by Illumina Miseq platform. The outcome of this study showed the genus of , and were significantly enriched in microorganism community composition. Further correlation analysis with soil pathogen ( TR4) content showed that was significantly negatively correlated with pathogen content. Therefore, we isolated and identified from the disease-suppressive soils, and obtained a strain YN1910. and pot experiments showed that YN1910 had a significant control effect (78.43-81.76%) on banana Fusarium wilt and had a significant growth promotion effect on banana plants.

由尖孢镰刀菌古巴专化型热带4号小种（TR4）引起的香蕉枯萎病，对香蕉的可持续生产构成了严重问题。生物防治是控制这种毁灭性病害的有效措施之一。土壤微生物的高通量测序能够显著提高生防菌株筛选的效率和准确性。在本研究中，利用Illumina Miseq平台对云南六个主要香蕉产区的土壤微生物多样性进行了测序。本研究结果表明，在微生物群落组成中，芽孢杆菌属、假单胞菌属和伯克氏菌属显著富集。进一步与土壤病原菌（TR4）含量进行相关性分析表明，芽孢杆菌属与病原菌含量显著负相关。因此，我们从抑病土壤中分离鉴定出芽孢杆菌属，并获得了一株芽孢杆菌菌株YN1910。温室和盆栽试验表明，YN1910对香蕉枯萎病具有显著的防治效果（78.43-81.76%），对香蕉植株具有显著的促生作用。