Feng Xinyu, Shi Qing, Jian Qiang, Li Fan, Li Zheng, Cheng Kang
Department of Cardiac and Pan-Vascular Diseases, Xi'an People's Hospital (Xi'an Fourth Hospital), Xi'an, China.
Xi'an Satellite Control Center, Xi'an, China.
Biochem Biophys Rep. 2023 Jul 4;35:101509. doi: 10.1016/j.bbrep.2023.101509. eCollection 2023 Sep.
The alterations in mitochondrial protein glycosylation in myocardial ischaemia reperfusion (I/R) injury are still unclear. Therefore, based on a lectin microarray and liquid chromatograph-mass spectrometer/mass spectrometer (LC‒MS/MS) technology combined with a bioinformatics analysis, we studied the changes in mitochondrial protein glycosylation during I/R injury. This study revealed significant differences in mitochondrial glycoprotein during I/R injury. Compared with the sham operation group, the model group, which underwent ischaemia for 30 min, showed a high expression of glycan structures recognized by lectins, such as WFA, PTL-I, LTL, GSL-I, SBA and SNA, and a low expression of glycan structures recognized by ConA, VVA and RCA120. The model group, which underwent ischaemia for 45 min, showed a high expression of glycan structures recognized by LTL and SNA and a low expression of glycan structures recognized by ECA. Further analysis showed that the Siaα2-6Gal/N-acetylgalactosamine (GalNAc) structures recognized by SNA were significantly increased. In total, 91 differential proteins were identified by LC‒MS/MS, and 8 hub genes were screened by Gene Ontology (GO) enrichment, Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment and protein interaction analyses. Compared with the Gene Expression Omnibus (GEO) database genes, two differential genes, Pros1 and Vtn, were obtained. Pros1 is a key regulator of the inflammatory response and vascular injury response. The Vtn gene variant is associated with the risk of myocardial infarction. This study is expected to provide a new method for the treatment of I/R injury and could provide new ideas for the postoperative prognosis of patients.
心肌缺血再灌注（I/R）损伤中线粒体蛋白糖基化的变化仍不清楚。因此，基于凝集素微阵列和液相色谱-质谱联用仪/质谱仪（LC-MS/MS）技术并结合生物信息学分析，我们研究了I/R损伤期间线粒体蛋白糖基化的变化。本研究揭示了I/R损伤期间线粒体糖蛋白的显著差异。与假手术组相比，缺血30分钟的模型组显示出凝集素识别的聚糖结构高表达，如WFA、PTL-I、LTL、GSL-I、SBA和SNA，而ConA、VVA和RCA120识别的聚糖结构低表达。缺血45分钟的模型组显示出LTL和SNA识别的聚糖结构高表达，而ECA识别的聚糖结构低表达。进一步分析表明，SNA识别的Siaα2-6Gal/N-乙酰半乳糖胺（GalNAc）结构显著增加。通过LC-MS/MS共鉴定出91种差异蛋白，并通过基因本体论（GO）富集、京都基因与基因组百科全书（KEGG）富集和蛋白质相互作用分析筛选出8个枢纽基因。与基因表达综合数据库（GEO）基因相比，获得了两个差异基因Pros1和Vtn。Pros1是炎症反应和血管损伤反应的关键调节因子。Vtn基因变异与心肌梗死风险相关。本研究有望为I/R损伤的治疗提供新方法，并可为患者术后预后提供新思路。
