Institut Pasteur, CNRS UMR 3525, Université Paris Cité, Unité Régulation Spatiale des Génomes, 75015, Paris, France.
Institut Pasteur, CNRS UMR 3525, Université Paris Cité, Unité Régulation Spatiale des Génomes, 75015, Paris, France.
Curr Opin Struct Biol. 2023 Oct;82:102679. doi: 10.1016/j.sbi.2023.102679. Epub 2023 Aug 19.
Chromosomes in all domains of life are well-defined structural entities with complex hierarchical organization. The regulation of this hierarchical organization and its functional interplay with gene expression or other chromosome metabolic processes such as repair, replication, or segregation is actively investigated in a variety of species, including prokaryotes. Bacterial chromosomes are typically gene-dense with few non-coding sequences and are organized into the nucleoid, a membrane-less compartment composed of DNA, RNA, and proteins (nucleoid-associated proteins or NAPs). The continuous improvement of imaging and genomic methods has put the organization of these Mb-long molecules at reach, allowing to disambiguate some of their highly dynamic properties and intertwined structural features. Here we review and discuss some of the recent advances in the field of bacterial chromosome organization.
所有生命领域的染色体都是具有复杂层次结构的明确结构实体。这种层次结构的调节及其与基因表达或其他染色体代谢过程（如修复、复制或分离）的功能相互作用，在包括原核生物在内的多种物种中得到了积极的研究。细菌染色体通常基因密度高，非编码序列少，并组织成类核，这是一个由 DNA、RNA 和蛋白质（类核相关蛋白或 NAPs）组成的无膜隔室。成像和基因组方法的不断改进，使得这些长达 Mb 的分子的组织得以实现，从而可以澄清它们的一些高度动态特性和交织的结构特征。在这里，我们回顾和讨论了细菌染色体组织领域的一些最新进展。
