Université Paris-Saclay, UVSQ, Inserm, Infection et inflammation, Montigny-Le-Bretonneux, France.
Methods Mol Biol. 2024;2819:3-26. doi: 10.1007/978-1-0716-3930-6_1.
The complex architecture of DNA within living organisms is essential for maintaining the genetic information that dictates their functions and characteristics. Among the many complexities of genetic material organization, the folding and arrangement of DNA into chromosomes play a critical role in regulating gene expression, replication, and other essential cellular processes. Bacteria, despite their apparently simple cellular structure, exhibit a remarkable level of chromosomal organization that influences their adaptability and survival in diverse environments. Understanding the three-dimensional arrangement of bacterial chromosomes has long been a challenge due to technical limitations, but the development of Chromosome Conformation Capture (3C) methods revolutionized our ability to explore the hierarchical structure and the dynamics of bacterial genomes. Here, we review the major advances in the field of bacterial chromosome structure using 3C technology over the past decade.
生物体内 DNA 的复杂结构对于维持决定其功能和特征的遗传信息至关重要。在遗传物质组织的众多复杂性中，DNA 折叠和排列成染色体在调节基因表达、复制和其他重要细胞过程中起着关键作用。尽管细菌的细胞结构看起来很简单，但它们表现出的染色体组织水平却非常显著，这影响了它们在不同环境中的适应性和生存能力。由于技术限制，长期以来，了解细菌染色体的三维排列一直是一个挑战，但染色体构象捕获（3C）方法的发展彻底改变了我们探索细菌基因组的层次结构和动态的能力。在这里，我们回顾了过去十年中使用 3C 技术在细菌染色体结构领域的主要进展。
