Boston University, United States of America.
Boston University School of Medicine, United States of America.
Prostaglandins Leukot Essent Fatty Acids. 2023 Oct;197:102586. doi: 10.1016/j.plefa.2023.102586. Epub 2023 Aug 10.
The purpose of this study is to investigate heart-fatty acid binding protein (H-FABP) leakage from cardiomyocytes as a quantitative measure of cell membrane damage and to test healing by Resolvin E1 (RVE1) as a potential therapeutic for patients with inflammatory diseases (cardiovascular disease and comorbidities) with high morbidity and mortality. Our quantitative ELISA assays demonstrated H-FABP as a sensitive and reliable biomarker for measuring cardiomyocyte damage induced by lipopolysaccharide (LPS) and healing by RvE1, a specialized pro-resolving mediator (SPM) derived from the Omega-3 fatty acid, eicosapentaenoic acid (EPA), a dietary nutrient that balances inflammation to restore homeostasis. RvE1 reduced leakage of H-FABP by up to 86%, which supports our hypothesis that inflammation as a mechanism of injury can be targeted for therapy. H-FABP as a blood biomarker was tested in 40 patients admitted to Boston Medical Center for respiratory distress, (20 patients with and 20 patients without COVID infection). High levels of H-FABP correlated with clinically diagnosed CVD, diabetes, and end-stage renal disease (ESRD) in both patient groups. The level of H-FABP indicates not only CVD damage but is a valuable measure for patients with increased inflammation disease comorbidities.
本研究旨在探讨心肌细胞中心脂肪酸结合蛋白（H-FABP）的渗漏情况，将其作为细胞膜损伤的定量指标，并通过 Resolvin E1（RVE1）的修复作用来测试其对患有炎症性疾病（心血管疾病和合并症）的患者的潜在治疗作用，此类患者具有高发病率和死亡率。我们的定量 ELISA 检测表明 H-FABP 是一种敏感且可靠的生物标志物，可用于测量脂多糖（LPS）诱导的心肌细胞损伤和 RvE1 的修复作用，RvE1 是一种来源于ω-3 脂肪酸二十碳五烯酸（EPA）的特殊促解决介质（SPM），是一种可平衡炎症、恢复体内平衡的膳食营养物。RvE1 可使 H-FABP 的渗漏减少多达 86%，这支持了我们的假设，即炎症作为一种损伤机制可以作为治疗的靶点。我们在因呼吸窘迫而入住波士顿医疗中心的 40 名患者中对 H-FABP 作为血液生物标志物进行了测试，（其中 20 名患者患有 COVID 感染，20 名患者未患有 COVID 感染）。在这两组患者中，H-FABP 水平与临床诊断的心血管疾病、糖尿病和终末期肾病（ESRD）均相关。H-FABP 水平不仅表明了心血管疾病的损害，而且是衡量炎症性疾病合并症患者的重要指标。
