School of Medicine, Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University, Shanghai, China,
National Clinical Research Center for Ophthalmic Diseases, Shanghai, China,
Gerontology. 2023;69(10):1218-1231. doi: 10.1159/000531287. Epub 2023 Aug 21.
Neovascular age-related macular degeneration (NVAMD) is a leading cause of severe vision impairment in the elderly. Aging is one of the most pivotal underlying molecular mechanisms of NVAMD.
In this study, we identified the potential aging-related genes involved in NVAMD. Considering that noncoding RNAs are vital regulators of NVAMD progression, we further explored and constructed an aging-originated circRNA-miRNA-mRNA network of NVAMD. Differential expression of 23 aging-associated genes was identified based on sequencing data and the Human Aging Genomic Resources tool at a threshold of p < 0.05, and log2|fold change| > 1.
We screened 12 microRNAs (miRNAs) using public datasets and miRNet database. A total of 13 circRNAs were subsequently mined using the starBase tool. Merging these 13 circRNAs, 12 miRNAs, and 15 genes together, we obtained 281 pairs of circRNA-miRNA and 30 pairs of miRNA-mRNA.
We created an aging-related circRNA-miRNA-mRNA network, which could be a promising target for future AMD treatments.
新生血管性年龄相关性黄斑变性（NVAMD）是老年人视力严重受损的主要原因。衰老时 NVAMD 最关键的潜在分子机制之一。
在这项研究中，我们鉴定了与 NVAMD 相关的潜在衰老相关基因。考虑到非编码 RNA 是 NVAMD 进展的重要调节因子，我们进一步探索并构建了 NVAMD 的衰老起源的 circRNA-miRNA-mRNA 网络。根据测序数据和 Human Aging Genomic Resources 工具，在 p < 0.05 和 log2|fold change|> 1 的阈值下，确定了 23 个与衰老相关的基因的差异表达。
我们使用公共数据集和 miRNet 数据库筛选了 12 个 microRNAs（miRNAs）。使用 starBase 工具随后挖掘了总共 13 个 circRNAs。将这 13 个 circRNAs、12 个 miRNAs 和 15 个基因合并在一起，我们获得了 281 对 circRNA-miRNA 和 30 对 miRNA-mRNA。
我们创建了一个与衰老相关的 circRNA-miRNA-mRNA 网络，这可能是未来 AMD 治疗的有前途的靶点。
