鉴定和验证新生血管性年龄相关性黄斑变性中与衰老相关的 circRNA-miRNA-mRNA 网络。

Identification and Validation of an Aging-Associated circRNA-miRNA-mRNA Network in Neovascular Age-Related Macular Degeneration.

机构信息

School of Medicine, Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University, Shanghai, China,

National Clinical Research Center for Ophthalmic Diseases, Shanghai, China,

出版信息

Gerontology. 2023;69(10):1218-1231. doi: 10.1159/000531287. Epub 2023 Aug 21.

INTRODUCTION

Neovascular age-related macular degeneration (NVAMD) is a leading cause of severe vision impairment in the elderly. Aging is one of the most pivotal underlying molecular mechanisms of NVAMD.

METHODS

In this study, we identified the potential aging-related genes involved in NVAMD. Considering that noncoding RNAs are vital regulators of NVAMD progression, we further explored and constructed an aging-originated circRNA-miRNA-mRNA network of NVAMD. Differential expression of 23 aging-associated genes was identified based on sequencing data and the Human Aging Genomic Resources tool at a threshold of p < 0.05, and log2|fold change| > 1.

RESULTS

We screened 12 microRNAs (miRNAs) using public datasets and miRNet database. A total of 13 circRNAs were subsequently mined using the starBase tool. Merging these 13 circRNAs, 12 miRNAs, and 15 genes together, we obtained 281 pairs of circRNA-miRNA and 30 pairs of miRNA-mRNA.

CONCLUSION

We created an aging-related circRNA-miRNA-mRNA network, which could be a promising target for future AMD treatments.

简介

新生血管性年龄相关性黄斑变性（NVAMD）是老年人视力严重受损的主要原因。衰老时 NVAMD 最关键的潜在分子机制之一。

方法

在这项研究中，我们鉴定了与 NVAMD 相关的潜在衰老相关基因。考虑到非编码 RNA 是 NVAMD 进展的重要调节因子，我们进一步探索并构建了 NVAMD 的衰老起源的 circRNA-miRNA-mRNA 网络。根据测序数据和 Human Aging Genomic Resources 工具，在 p < 0.05 和 log2|fold change|> 1 的阈值下，确定了 23 个与衰老相关的基因的差异表达。

结果

我们使用公共数据集和 miRNet 数据库筛选了 12 个 microRNAs（miRNAs）。使用 starBase 工具随后挖掘了总共 13 个 circRNAs。将这 13 个 circRNAs、12 个 miRNAs 和 15 个基因合并在一起，我们获得了 281 对 circRNA-miRNA 和 30 对 miRNA-mRNA。