State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
Bioresour Technol. 2023 Nov;387:129702. doi: 10.1016/j.biortech.2023.129702. Epub 2023 Aug 19.
This study proposes a new model in which ethanol and acetate produced by dark fermentation are processed by Clostridium kluyveri for chain elongation to produce caproate with an addition of biochar prepared from cornstalk residues after acid pretreatment and enzymatic hydrolysis (AERBC) in the dark fermentation and chain elongation processes. The results show a 6-25% increase in hydrogen production in dark fermentation with adding AERBC, and the maximum concentration of caproate in the new model reached 1740 mg/L, 61% higher than that in the control group. In addition, caproate was obtained by dark fermentation, using liquid metabolites as substrates with an initial pH range of 6.5-7.5. Finally, the electron balance and electron transfer efficiency in the new model were analyzed, and the role of AERBC in dark fermentation and chain elongation was investigated. This study provides a new reference for the use of dark-fermented liquid metabolites and cornstalk residue.
本研究提出了一个新的模型，即在暗发酵过程中产生的乙醇和乙酸通过克氏梭菌进行链延长，以生产己酸，添加的生物炭是由酸预处理和酶解后的玉米秸秆残渣（AERBC）制备的，可在暗发酵和链延长过程中使用。结果表明，在暗发酵过程中添加 AERBC 可使氢气产量提高 6-25%，新模型中己酸的最大浓度达到 1740mg/L，比对照组高 61%。此外，己酸是通过暗发酵获得的，使用初始 pH 值范围为 6.5-7.5 的液体代谢物作为底物。最后，分析了新模型中的电子平衡和电子传递效率，并研究了 AERBC 在暗发酵和链延长中的作用。本研究为暗发酵液体代谢物和玉米秸秆残渣的利用提供了新的参考。
