Department of Engineering, University of Campania "Luigi Vanvitelli", Via Roma 29, 81031 Aversa, Italy; Institute of Sustainable Processes, University of Valladolid, Dr. Mergelina, s/n, 47011 Valladolid, Spain.
Department of Engineering, University of Campania "Luigi Vanvitelli", Via Roma 29, 81031 Aversa, Italy.
Bioresour Technol. 2023 Nov;387:129699. doi: 10.1016/j.biortech.2023.129699. Epub 2023 Aug 19.
In this work, the potential of Methylocystis hirsuta to simultaneously use methane and volatile fatty acids mixtures for triggering PHBV accumulation was assessed for the first time batchwise. Biotic controls carried out with CH alone confirmed the inability of Methylocystis hirsuta to produce PHBV and achieved 71.2 ± 7 g md of PHB. Pure valeric acid and two synthetic mixtures simulating VFAs effluents from the anaerobic digestion of food waste at 35 °C (M) and 55 °C (M) were supplied to promote 3-HV inclusion. Results showed that pure valeric acid supported the highest polymer yields of 105.8 ± 9 g md (3-HB:3-HV=70:30). M mixtures led to a maximum of 103 ± 4 g md of PHBV (3-HB:3-HV=85:15), while M mixtures, which did not include valeric acid, showed no PHV synthesis. This suggested that the synthesis of PHBV from VFAs effluents depends on the composition of the mixtures, which can be tuned during the anaerobic digestion process.
在这项工作中，首次分批评估了丝状藻属（Methylocystis hirsuta）同时利用甲烷和挥发性脂肪酸混合物来触发 PHBV 积累的潜力。仅用 CH 进行的生物对照实验证实了丝状藻属不能产生 PHBV，并实现了 71.2±7 gmd 的 PHB。纯戊酸和两种模拟模拟食品废物厌氧消化在 35°C（M）和 55°C（M）下产生的 VFAs 流出物的混合物被供应以促进 3-HV 包含。结果表明，纯戊酸支持最高的聚合物产率为 105.8±9 gmd（3-HB:3-HV=70:30）。M 混合物导致最高 103±4 gmd 的 PHBV（3-HB:3-HV=85:15），而不含戊酸的 M 混合物则没有 PHV 合成。这表明从 VFAs 流出物合成 PHBV 取决于混合物的组成，这可以在厌氧消化过程中进行调整。
