Amabile Claudia, Abate Teresa, Marcos Enrique, Chianese Simeone, Musmarra Dino, Muñoz Raul
Department of Engineering, University of Campania "Luigi Vanvitelli", Via Roma 29, Aversa 81031, Italy.
Institute of Sustainable Processes, University of Valladolid, Dr. Mergelina, s/n, Valladolid 47011, Spain.
ACS Sustain Chem Eng. 2024 Mar 4;12(11):4690-4699. doi: 10.1021/acssuschemeng.3c08570. eCollection 2024 Mar 18.
In this work, the potential of a synthetic coculture and a mixed methanotrophic consortium to synthesize poly(3-hydroxybutyrate--3-hydroxyvalerate) (PHBV) from renewable and waste-based feedstocks was assessed batchwise. cocultivated with and a -enriched culture previously grown on methane were subjected to nutrient starvation in a medium enriched with valeric acid (30% w w of ) or with a VFAs mixture containing acetic, propionic, butyric, and valeric acids (15% w w of ) under a CH:O or air atmosphere. For all test series, pH was adjusted to 7 after adding the cosubstrates, and a negligible substrate consumption or polymer production was considered the end point of the trial. Results showed that valeric acid promoted PHBV accumulation in both cultures regardless of the atmosphere. Interestingly, the mixture of VFAs supported PHBV accumulation only in the presence of methane. The highest PHBV contents for the coculture and the mixed consortium, equal to 73.7 ± 2.5% w w and 49.6 ± 13% w w, respectively, were obtained with methane and the VFAs mixture. This study demonstrates the suitability of cocultures and biobased cosubstrates for the sustainable production of the biodegradable polymer PHBV.
在这项工作中，分批评估了合成共培养物和混合甲烷营养菌群从可再生和废弃原料合成聚（3-羟基丁酸酯-3-羟基戊酸酯）（PHBV）的潜力。将与先前在甲烷上生长的富集培养物共培养的菌株在富含戊酸（占底物的30% w/w）或含有乙酸、丙酸、丁酸和戊酸的挥发性脂肪酸混合物（占底物的15% w/w）的培养基中，在CH₄:O₂或空气气氛下进行营养饥饿处理。对于所有测试系列，在添加共底物后将pH值调至7，底物消耗或聚合物产量可忽略不计被视为试验终点。结果表明，无论在何种气氛下，戊酸均能促进两种培养物中PHBV的积累。有趣的是，挥发性脂肪酸混合物仅在有甲烷存在时才支持PHBV的积累。共培养物和混合菌群的最高PHBV含量分别为73.7 ± 2.5% w/w和49.6 ± 13% w/w，是在甲烷和挥发性脂肪酸混合物存在的情况下获得的。本研究证明了共培养物和生物基共底物对于可持续生产可生物降解聚合物PHBV的适用性。
