Instituto Federal de Educação, Ciência e Tecnologia Goiano, Campus Rio Verde, Goiás, Brazil.
Universidade Federal de Uberlândia, Laboratório de Ensaios Antimicrobiano (LEA), Uberlândia, Minas Gerais, Brazil.
Nat Prod Res. 2024 Oct;38(19):3476-3480. doi: 10.1080/14786419.2023.2249584. Epub 2023 Aug 22.
Citrus canker, which is caused by , is a severe disease that affects citrus plants worldwide. This paper aimed to compare, for the first time, the chemical composition and anti- activities of essential oils from fresh and dry leaves (EO-FL and EO-DL, respectively). Anti- activity of spathulenol, the major constituent of oils, was also evaluated. Activities were screened by the broth microdilution method on 96-well culture plates. Three major constituents were identified in EO-FL and EO-DL by GC-MS and GC-FID: spathulenol, β-caryophyllene and caryophyllene oxide. EO-DL (MIC = 31.25 µg/mL), EO-FL (MIC = 62.5 µg/mL) and spathulenol (MIC = 100 µg/mL) were active against strains (resistant, tolerant and sensitive to copper). Even though results showed that potential of EO-FL, EO-DL and spathulenol against , further studies are needed to prove their applicability to the biocontrol of citrus canker.
溃疡病，由 引起，是一种严重的疾病，影响全球的柑橘植物。本研究旨在首次比较新鲜和干燥叶片（EO-FL 和 EO-DL）精油的化学成分和抗 活性。还评估了油中主要成分 - 匙叶桉醇的抗 活性。抗 活性通过 96 孔培养板的肉汤微量稀释法进行筛选。通过 GC-MS 和 GC-FID 在 EO-FL 和 EO-DL 中鉴定出三种主要成分：匙叶桉醇、β-石竹烯和石竹烯氧化物。EO-DL（MIC = 31.25 µg/mL）、EO-FL（MIC = 62.5 µg/mL）和匙叶桉醇（MIC = 100 µg/mL）对 菌株（对铜具有抗性、耐受性和敏感性）具有活性。尽管结果表明 EO-FL、EO-DL 和匙叶桉醇对 具有潜力，但仍需要进一步的研究来证明它们在柑橘溃疡病的生物防治中的适用性。
