Unité de recherche M.A.C.S. Ecole Natl. d'Ingénieurs de Gabès, Gabès, Tunisia.
J Food Sci. 2010 Aug 1;75(6):C466-72. doi: 10.1111/j.1750-3841.2010.01711.x.
Essential oils were obtained by steam distillation from berries of Schinus molle L. and Schinus terebinthifolius Raddi originating from southern of Tunisia and analyzed by GC-FID and GC-MS. Among 57 and 62 compounds (%[mg/100 g dry matter]) identified in these oils, the main were alpha-phellandrene (46.52%[1256.15] and 34.38%[859.60]), beta-phellandrene (20.81%[561.74] and 10.61%[265.15]), alpha-terpineol (8.38%[226.26] and 5.60%[140.03]), alpha-pinene (4.34%[117.29] and 6.49%[162.25]), beta-pinene (4.96%[133.81] and 3.09%[77.30]) and p-cymene (2.49%[67.28] and 7.34%[183.40]), respectively. A marked quantity of gamma-cadinene (18.04%[451.05]) was also identified in the S. terebinthifolius essential oil whereas only traces (0.07%[1.81]) were detected in the essential oil of S. molle. The in vitro antioxidant and antiradical scavenging properties of the investigated essential oils were evaluated by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-Azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays. Essential oil of S. terebinthifolius expressed stronger antioxidant activity in the ABTS assay, with an IC(50) of 24 +/- 0.8 mg/L, compared to S. molle (IC(50)= 257 +/- 10.3 mg/L). Essential oils were also evaluated for their anticancer activities against human breast cancer cells (MCF-7). S. terebinthifolius essential oil was more effective against tested cell lines (IC(50)= 47 +/- 9 mg/L) than that from S. molle (IC(50)= 54 +/- 10 mg/L). Suggestions on relationships between chemical composition and biological activities are outlined.
从突尼斯南部产的 Schinus molle L. 和 Schinus terebinthifolius Raddi 的浆果中通过水蒸气蒸馏法获得精油，并通过 GC-FID 和 GC-MS 进行分析。在这些油中鉴定出的 57 种和 62 种化合物（%[mg/100 g 干物质]）中，主要的是α-水芹烯（46.52%[1256.15]和 34.38%[859.60]）、β-水芹烯（20.81%[561.74]和 10.61%[265.15]）、α-萜品醇（8.38%[226.26]和 5.60%[140.03]）、α-蒎烯（4.34%[117.29]和 6.49%[162.25]）、β-蒎烯（4.96%[133.81]和 3.09%[77.30]）和对伞花烃（2.49%[67.28]和 7.34%[183.40]）。在 S. terebinthifolius 的精油中还鉴定出大量的γ-杜松烯（18.04%[451.05]），而 S. molle 的精油中仅检测到微量（0.07%[1.81]）。通过使用 1,1-二苯基-2-苦基肼（DPPH）和 2,2'-联氮双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）测定法评估了所研究的精油的体外抗氧化和清除自由基活性。S. terebinthifolius 的精油在 ABTS 测定中表现出更强的抗氧化活性，其 IC50 为 24 +/- 0.8 mg/L，而 S. molle 的 IC50 为 257 +/- 10.3 mg/L。还评估了精油对人乳腺癌细胞（MCF-7）的抗癌活性。S. terebinthifolius 的精油对测试细胞系的作用更有效（IC50=47 +/- 9 mg/L），而 S. molle 的精油的作用较弱（IC50=54 +/- 10 mg/L）。概述了化学组成与生物活性之间的关系的建议。
