Yang Zhongchen, Lou Yanhong, Yan Xianghui, Pan Hong, Wang Hui, Yang Quangang, Sun Yajie, Zhuge Yuping
National Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources, College of Resources and Environment, Shandong Agricultural University, Tai'an 271018, China.
J Xenobiot. 2023 Aug 15;13(3):424-438. doi: 10.3390/jox13030027.
The decomposition of biodegradable composite carbon sources generates a large amount of biodegradable microplastics, which may not only furnish microbial denitrification, but might also pose potential environmental risks. In the present study, the effects of different dosages of a biodegradable composite carbon source on the microbial communities, the nitrogen metabolic pathways and the antibiotic resistome were explored through Illumina MiSeq sequencing analysis and metagenomic analysis. The results of partial least-square discriminant analysis (PLS-DA) and analysis of similarity (ANOSIM) demonstrated that the response of the bacterial community to a biodegradable composite carbon source was more obvious than the fungal community. The application of biodegradable microplastics diminished the complexity of the microbial communities to some extent and obviously stimulated denitrification. Antibiotics resistance gene (ARG) dispersal was not evidently accelerated after the addition of biodegradable composite carbon source. , , , , from and , , and from were the major ARG hosts. Overall, the addition of a biodegradable composite carbon source shaped microbial communities and their antibiotic resistance profiles in this study.
可生物降解复合碳源的分解会产生大量可生物降解微塑料，这些微塑料不仅可能为微生物反硝化作用提供条件，还可能带来潜在的环境风险。在本研究中，通过Illumina MiSeq测序分析和宏基因组分析，探究了不同剂量的可生物降解复合碳源对微生物群落、氮代谢途径和抗生素抗性组的影响。偏最小二乘判别分析（PLS-DA）和相似性分析（ANOSIM）结果表明，细菌群落对可生物降解复合碳源的响应比真菌群落更明显。可生物降解微塑料的应用在一定程度上降低了微生物群落的复杂性，并明显促进了反硝化作用。添加可生物降解复合碳源后，抗生素抗性基因（ARG）的扩散并未明显加速。来自[具体来源1]的[具体基因1]、[具体基因2]、[具体基因3]以及来自[具体来源2]的[具体基因4]、[具体基因5]、[具体基因6]是主要的ARG宿主。总体而言，在本研究中，可生物降解复合碳源的添加塑造了微生物群落及其抗生素抗性谱。
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