Kudolo G B, Mbai F N, Eley R M
J Endocrinol. 1986 Sep;110(3):429-39. doi: 10.1677/joe.0.1100429.
Sixteen individually caged adult female vervet monkeys (Cercopithecus aethiops), whose reproductive parameters had been studied for 5 years, were hysterectomized/ovariectomized during three reproductive states; i.e. the late follicular (15.4 +/- 4.7 (S.D.) days) and luteal (27.8 +/- 4.7 days) phases of the normal cycle (20-50 days), and during prolonged intermenstrual intervals (PII; 99.0 +/- 2.5 days since the previous menses). These latter animals showed characteristics of both follicular and luteal phases; i.e. their ovaries contained both corpora lutea and large antral follicles and systemic oestradiol and progesterone concentrations were raised. Analysis of cytoplasmic oestrogen and progestin receptors (CER and CPR) revealed that endometrium during PII had CER levels of 0.58 +/- 0.07 pmol/mg protein, similar to those of the follicular phase (0.60 +/- 0.12); CPR levels (1.20 +/- 0.87) were not different from those of the luteal phase (1.05 +/- 0.45). The ratio of CPR to CER during the luteal phase was about tenfold higher than that of the follicular phase. Levels during PII were intermediate between the two phases. Under receptor-activating conditions, the DNA-binding components of the PII cytoplasmic fraction underwent over 40% loss while those present during both phases of the normal cycle doubled. The hormone-binding sites at all times remained intact indicating that the DNA and hormone-binding sites are distinct on both CER and CPR. Less than 50% interaction of CER/CPR with DNA-cellulose was obtained, indicating the presence of only limited quantities of cytoplasmic activating factors which may be a prerequisite for receptor binding to the genome. During PII, factors which deactivate DNA-binding sites may also have been induced. Extensive accumulation of nuclear oestrogen receptor was evident in PII endometrium with 80% being salt-resistant. This level is higher than that in the follicular and luteal phases (37 and 52% respectively). These data, suggesting a possible aberration of receptor activation in vitro and receptor processing in vivo, may be indicative of endometrial dysfunction during PII. This could lead to a delay in menstruation.
16只单独关养的成年雌性黑长尾猴（猕猴属埃塞俄比亚种），其生殖参数已研究了5年，在三个生殖状态下进行了子宫切除/卵巢切除手术；即正常周期（20 - 50天）的卵泡晚期（15.4±4.7（标准差）天）和黄体期（27.8±4.7天），以及在月经间期延长（PII；自上次月经以来99.0±2.5天）期间。后一组动物表现出卵泡期和黄体期的特征；即它们的卵巢中既有黄体又有大的卵泡腔卵泡，且全身雌二醇和孕酮浓度升高。对细胞质雌激素和孕激素受体（CER和CPR）的分析显示，PII期间子宫内膜的CER水平为0.58±0.07 pmol/mg蛋白，与卵泡期（0.60±0.12）相似；CPR水平（1.20±0.87）与黄体期（1.05±0.45）无差异。黄体期CPR与CER的比值比卵泡期高约十倍。PII期间的水平介于两个阶段之间。在受体激活条件下，PII细胞质部分的DNA结合成分损失超过40%，而正常周期两个阶段的DNA结合成分则增加一倍。激素结合位点在所有时间都保持完整，表明CER和CPR上的DNA和激素结合位点是不同的。CER/CPR与DNA - 纤维素的相互作用不到50%，表明仅存在有限数量的细胞质激活因子，这可能是受体与基因组结合的先决条件。在PII期间，也可能诱导了使DNA结合位点失活的因子。PII子宫内膜中核雌激素受体大量积累，80%为耐盐型。这个水平高于卵泡期和黄体期（分别为37%和52%）。这些数据表明体外受体激活和体内受体加工可能存在异常，可能表明PII期间子宫内膜功能障碍。这可能导致月经推迟。
