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与母鼠尼古丁暴露相关的基因表达谱在其子代鼠肝脏中的表现。

The Gene Expression Profiles Associated with Maternal Nicotine Exposure in the Liver of Offspring Mice.

机构信息

Department of Ultrasound, The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, China.

Department of Ultrasound, Fujian Maternity and Child Health Hospital College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Fuzhou, China.

出版信息

Reprod Sci. 2024 Jan;31(1):212-221. doi: 10.1007/s43032-023-01328-3. Epub 2023 Aug 22.

Abstract

This study aims to investigate the effect of maternal nicotine exposure on the gene expression profiles in the liver of offspring mice. Pregnant mice were subcutaneously injected with either saline vehicle or nicotine twice a day on gestational days 11-21. Total RNA from the liver samples which collected from the offspring mice of postnatal day 7 and 21 was subjected to RNA sequencing. Gene Ontology (GO) functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) signaling pathway enrichment analysis were conducted to identify the functions of differentially expressed genes (DEGs). Four genes were selected for further validation by quantitative reverse transcription polymerase chain reaction (qRT-PCR). A total of 448 DEGs and 186 DEGs were identified on postnatal day 7 and 21, respectively. GO analysis revealed that the DEGs on postnatal day 7 mainly participated in the biological functions of cell growth and proliferation, and the DEGs on postnatal day 21 mainly participated in ion transport/activity. KEGG enrichment analysis showed that the DEGs on postnatal day 7 were mainly enriched in the cell cycle, cytokine-cytokine receptor interactions, hypertrophic cardiomyopathy, and the p53 signaling pathway, while the DEGs on postnatal day 21 were mainly enriched in neuroactive ligand-receptor interactions, the calcium signaling pathway, retinol metabolism, and axon guidance. The qRT-PCR results were consistent with the RNA sequencing data. The DEGs may affect the growth of liver in early postnatal period while may affect ion transport/activity in late postnatal period.

摘要

本研究旨在探讨母体尼古丁暴露对仔鼠肝脏基因表达谱的影响。妊娠小鼠于妊娠第 11-21 天每天两次皮下注射生理盐水或尼古丁。收集出生后第 7 天和第 21 天仔鼠的肝脏样本总 RNA,进行 RNA 测序。通过基因本体 (GO) 功能富集分析和京都基因与基因组百科全书 (KEGG) 信号通路富集分析,鉴定差异表达基因 (DEGs) 的功能。选择 4 个基因进行定量逆转录聚合酶链反应 (qRT-PCR) 进一步验证。分别在出生后第 7 天和第 21 天鉴定出 448 个 DEGs 和 186 个 DEGs。GO 分析表明,出生后第 7 天的 DEGs 主要参与细胞生长和增殖的生物学功能,出生后第 21 天的 DEGs 主要参与离子转运/活性。KEGG 富集分析表明,出生后第 7 天的 DEGs 主要富集在细胞周期、细胞因子-细胞因子受体相互作用、肥厚型心肌病和 p53 信号通路,而出生后第 21 天的 DEGs 主要富集在神经活性配体-受体相互作用、钙信号通路、视黄醇代谢和轴突导向。qRT-PCR 结果与 RNA 测序数据一致。DEGs 可能影响仔鼠早期肝脏生长,而在后期可能影响离子转运/活性。

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