Smigocki A C, Voll M J
J Gen Microbiol. 1986 Apr;132(4):1027-33. doi: 10.1099/00221287-132-4-1027.
Transmissible factors encoding production of lacunae (L factors) were demonstrated in a non-O1 Vibrio cholerae and a Vibrio sp. of recent environmental origin. Lacunae were produced in lawns of non-O1 V. cholerae indicator strains under the same assay conditions as those where lacunae were produced by the well characterized P fertility plasmid of V. cholerae O1 and the V fertility factor found in a non-cholera vibrio strain. The origin of the lacunae produced by strains harbouring the V and L factors was examined. No vibriocin or phage activity was found in culture supernates or in lacunae produced by the strains, suggesting that, as in the case of the P plasmid, the lacunae probably represent sites of active mating. Unlike the P plasmid, neither the Vn or L factor could be detected or isolated by conventional plasmid techniques.
编码腔隙产生的可传播因子（L因子）在一株非O1群霍乱弧菌和一株近期来源于环境的弧菌属细菌中得到证实。在与由霍乱弧菌O1群特征明确的P致育质粒以及在一株非霍乱弧菌中发现的V致育因子产生腔隙相同的检测条件下，非O1群霍乱弧菌指示菌株的菌苔中产生了腔隙。对携带V因子和L因子的菌株产生的腔隙的来源进行了研究。在菌株产生的培养上清液或腔隙中未发现弧菌素或噬菌体活性，这表明，与P质粒的情况一样，腔隙可能代表活跃交配的位点。与P质粒不同，Vn因子和L因子均不能通过传统质粒技术检测到或分离出来。
