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霍乱弧菌非O1菌株中编码耐热直接溶血素的质粒携带基因的证明。

Demonstration of a plasmid-borne gene encoding a thermostable direct hemolysin in Vibrio cholerae non-O1 strains.

作者信息

Honda T, Nishibuchi M, Miwatani T, Kaper J B

出版信息

Appl Environ Microbiol. 1986 Nov;52(5):1218-20. doi: 10.1128/aem.52.5.1218-1220.1986.

DOI:10.1128/aem.52.5.1218-1220.1986
PMID:3789717
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC239203/
Abstract

Of 15 Vibrio cholerae non-O1 strains, 2 produced a hemolysin termed NAG-rTDH, which is very similar to the thermostable direct hemolysin of V. parahaemolyticus. These two strains contained DNA sequences which are homologous to a DNA probe for the V. parahaemolyticus thermostable direct hemolysin gene. A probe-positive 9-kilobase HindIII fragment was cloned from a plasmid of a V. cholerae non-O1 strain into plasmid pBR322, and the resulting Escherichia coli clones produced intracellular NAG-rTDH.

摘要

在15株霍乱弧菌非O1菌株中，有2株产生了一种名为NAG-rTDH的溶血素，它与副溶血性弧菌的耐热直接溶血素非常相似。这两株菌株含有与副溶血性弧菌耐热直接溶血素基因的DNA探针同源的DNA序列。从一株霍乱弧菌非O1菌株的质粒中克隆出一个探针阳性的9千碱基HindIII片段，将其插入质粒pBR322中，得到的大肠杆菌克隆产生细胞内NAG-rTDH。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40b8/239203/3e6208416087/aem00134-0253-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40b8/239203/b142a8256e8c/aem00134-0252-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40b8/239203/fc0d73336003/aem00134-0253-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40b8/239203/3e6208416087/aem00134-0253-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40b8/239203/b142a8256e8c/aem00134-0252-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40b8/239203/fc0d73336003/aem00134-0253-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40b8/239203/3e6208416087/aem00134-0253-b.jpg

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