Nordmann J J, Cazalis M
J Neurochem. 1986 Nov;47(5):1534-43. doi: 10.1111/j.1471-4159.1986.tb00791.x.
Neurosecretory granules from the rat and bovine neurohypophysis were isolated and some of their biochemical and biophysical properties studied. Neurosecretory granules (NSG) from rat neurohypophysis were labeled, in vivo, with [35S]cysteine and isolated on isoosmotic gradients. Whereas 1 day after labeling most of the radioactivity was found in the lower part of the gradient, 35 days later the isotope was also located in the lighter NSG-containing fraction. Different analytical procedures showed that the lighter fraction, both in bovine and rat NSG, contain more subpopulations of neurophysin-like material than the heavier fraction. The first material to be released during stimulation of secretion, in vivo or in vitro, is mobilized from the heavy NSG. Isolation of rat NSG, at different times during and after dehydration of the animals, reveals that the newly synthesized material is found in the heavy NSG-containing fraction. Furthermore, the results indicate that the newly synthesized NSG are more resistant to lysis than the lighter granules. The results are discussed in relation to the maturation and degradation processes of the granule content and to the functional state of the NSG.
从大鼠和牛的神经垂体中分离出神经分泌颗粒，并对其一些生化和生物物理特性进行了研究。用[35S]半胱氨酸在体内标记大鼠神经垂体的神经分泌颗粒，并通过等渗梯度进行分离。标记后1天，大部分放射性出现在梯度的下部，而35天后，该同位素也位于较轻的含神经分泌颗粒部分。不同的分析方法表明，无论是牛还是大鼠的神经分泌颗粒，较轻的部分比较重的部分含有更多种类的神经垂体素样物质。在体内或体外刺激分泌过程中首先释放的物质是从较重的神经分泌颗粒中动员出来的。在动物脱水期间及之后的不同时间分离大鼠神经分泌颗粒，结果显示新合成的物质存在于含较重神经分泌颗粒的部分。此外，结果表明新合成的神经分泌颗粒比较轻的颗粒更耐裂解。结合颗粒内容物的成熟和降解过程以及神经分泌颗粒的功能状态对这些结果进行了讨论。
