Nordmann J J
Neuroendocrinology. 1985 Jan;40(1):25-32. doi: 10.1159/000124047.
Neurohypophyses from rats dehydrated and rehydrated for different periods of time have been analyzed by morphometry of electron micrographs and their oxytocin and vasopressin contents measured by radioimmunoassays. The time course of disappearance and reappearance of neurosecretory granules (NSG) parallels that of the hormone content of the neurohypophysis. Depletion of NSG during dehydration first occurs in the nerve endings and only later in the swellings. No change of the volumetric density of the microvesicles was detected but the time course of changes in the volumetric density of the endocytotic vacuoles varies according to the secretory state of the tissue. There was a significant increase in the volume of the gland 3 days after the beginning of the rehydration. This increase was associated with an increase in the volumetric density of the pituicytes. No change of the total number of endings, swellings and axons was observed. This demonstrates a hypertrophy of the pituicytes. The present work gives strong support to the concept of a preferential release of the granules located in the endings. It also shows that, after depletion of the NSG in the neural lobe, newly synthetized granules at the onset of rehydration move first to the endings and 2 days later arrive in the swellings coming presumably either directly from the hypothalamus or from the endings. Furthermore, the results confirm previous work which has demonstrated that membrane retrieval in neurohypophysial nerve endings occurs via vacuoles.
对不同脱水和再水化时间段的大鼠神经垂体进行了电子显微镜形态计量分析，并通过放射免疫分析法测定了其催产素和加压素含量。神经分泌颗粒（NSG）消失和重新出现的时间进程与神经垂体激素含量的时间进程平行。脱水过程中NSG的耗竭首先发生在神经末梢，随后才发生在膨体中。未检测到微泡体积密度的变化，但内吞泡体积密度的变化时间进程因组织的分泌状态而异。再水化开始3天后腺体体积显著增加。这种增加与垂体细胞体积密度的增加有关。未观察到末梢、膨体和轴突总数的变化。这表明垂体细胞肥大。本研究为位于末梢的颗粒优先释放的概念提供了有力支持。它还表明，神经叶中NSG耗尽后，再水化开始时新合成的颗粒首先移动到末梢，2天后到达膨体，推测可能直接来自下丘脑或来自末梢。此外，结果证实了先前的研究，即神经垂体神经末梢的膜回收是通过空泡进行的。
