Halpin K M, Chausow D G, Baker D H
J Nutr. 1986 Sep;116(9):1747-51. doi: 10.1093/jn/116.9.1747.
Experiments were conducted with young chicks to quantify the absorption efficiency of manganese (Mn) using tibia Mn uptake as the response criterion. Chicks in experiment 1 were fed a corn-soybean meal diet and either injected intraperitoneally or crop intubated twice daily with three levels of inorganic Mn provided as MnSO4 X H2O. After 14 d, chicks were killed, and intact tibias were removed to facilitate calculation of bone Mn concentration. Excellent straight-line fits (bone Mn concentration as a function of Mn administered) allowed estimation of gut absorption efficiency of Mn by slope-ratio methodology. Absorption efficiency of Mn was calculated to be 1.71% for chicks fed the corn-soy diet. Identical methodology was employed in experiment 2 wherein chicks were fed a phytate- and fiber-free casein-dextrose diet. An absorption efficiency of 2.40% was estimated for chicks fed this diet. Hence, absorption efficiency of inorganic Mn was 40% greater in chicks fed the purified diet than in those fed the conventional corn-soy diet.
用幼雏进行实验,以胫锰摄取量作为反应标准来量化锰(Mn)的吸收效率。实验1中的雏鸡饲喂玉米-豆粕日粮,每天通过腹腔注射或嗉囊插管两次,提供三种水平的无机锰,以硫酸锰·水合物的形式。14天后,雏鸡被处死,取出完整的胫骨以方便计算骨锰浓度。极好的直线拟合(骨锰浓度作为所给锰的函数)使得通过斜率比方法估计锰的肠道吸收效率成为可能。对于饲喂玉米-豆日粮的雏鸡,计算出锰的吸收效率为1.71%。实验2采用相同的方法,其中雏鸡饲喂无植酸和纤维的酪蛋白-葡萄糖日粮。对于饲喂该日粮的雏鸡,估计吸收效率为2.40%。因此,饲喂纯化日粮的雏鸡对无机锰的吸收效率比饲喂传统玉米-豆粕日粮的雏鸡高40%。