Department of Radiology, The First Affiliated Hospital of Jinzhou Medical University, No. 2, Section 5, Renmin Street, Guta District, Jinzhou, 121000, Liaoning, China.
Biochem Genet. 2024 Apr;62(2):1365-1378. doi: 10.1007/s10528-023-10479-6. Epub 2023 Aug 23.
As one of the most frequent intracranial tumors, glioma showed invasive development and poor prognosis. lncRNAs have been illustrated to serve as biomarkers in various cancers. Whether the long non-coding RNA Prader Willi/Angelman region RNA 6 (PWAR6) was involved in glioma development and the underlying mechanism was investigated. PWAR6 in glioma was evaluated by polymerase chain reaction and its clinical significance was assessed with a series of statistical analyses. The biological function of PWAR6 was investigated with the cell counting kit 8 and Transwell assay. The potential underlying mechanism was studied with the luciferase reporter assay. The significant downregulation of PWAR6 was observed in glioma, which showed a close relationship with the major clinicopathological features and poor prognosis of patients. PWAR6 restrained cell growth, migration and invasion of glioma, which was alleviated by the overexpression of microRNA-106a-5p (miR-106a-5p). PWAR6 functioned as a prognostic biomarker and tumor suppressor of glioma through regulating miR-106a-5p.
作为最常见的颅内肿瘤之一，神经胶质瘤表现出侵袭性发展和预后不良。长链非编码 RNA 已被证明在各种癌症中可作为生物标志物。长非编码 RNA 普瑞德威利/安格曼综合征区域 RNA 6（PWAR6）是否参与神经胶质瘤的发生及其潜在机制尚待研究。采用聚合酶链反应检测神经胶质瘤中的 PWAR6，并通过一系列统计学分析评估其临床意义。采用细胞计数试剂盒 8 和 Transwell 检测研究 PWAR6 的生物学功能。采用荧光素酶报告基因检测研究潜在的作用机制。结果显示，PWAR6 在神经胶质瘤中显著下调，与患者的主要临床病理特征和不良预后密切相关。PWAR6 抑制神经胶质瘤细胞的生长、迁移和侵袭，而过表达 microRNA-106a-5p（miR-106a-5p）可减轻这种抑制作用。PWAR6 通过调节 miR-106a-5p 发挥神经胶质瘤的预后生物标志物和肿瘤抑制因子的作用。
