lncRNA sponges and inhibits BDNF/ERK pathway to suppress glioma cell proliferation and migration.

OBJECTIVE

Gliomas as primary cerebral malignancies frequently occurring in adults have relatively high morbidity and mortality. The underlying role of long non-coding ribonucleic acids (lncRNAs) in malignancies has attracted much attention, among which tumor suppressor candidate 7 () is a novel tumor suppressor gene whose regulatory mechanism in human cerebral gliomas remains inconclusive.

METHODS AND RESULTS

In this study, bioinformatics analysis indicated that could specifically bind to microRNA (miR)-10a-5p, and according to quantitative polymerase chain reaction (q-PCR), was up-regulated in human glioma cells and negatively correlated with expression. Dual-luciferase reporter gene assay showed the ability of to bind to , and overexpression of notably inhibited expression, restrained human glioma cell proliferation and migration, and regulated cell cycle and cyclin expression via the brain-derived neurotrophic factor/extracellular signal-regulated kinase (BDNF/ERK) pathway. The inhibitory effect of on was also verified by designing overexpression and knockdown panels for wound healing, Transwell and Western blotting assays.

CONCLUSIONS

suppresses human glioma cell proliferation and migration by negatively modulating and inhibiting the BDNF/ERK pathway, thus acting as a tumor suppressor gene in human gliomas.